Microscale methods to assemble mammalian cells into tissue-like structures  

作　　者：GONG PeiYuan ZHENG WenFu XIAO Dan JIANG XingYu 

机构地区：[1]College of Chemistry,Sichuan University,Chengdu 610064,China [2]Key Lab for Biological Effects of Nanomaterials and Nanosafety,National Center for Nanoscience and Technology,Beijing 100190,China

出　　处：《Science China(Life Sciences)》2012年第10期862-871,共10页中国科学（生命科学英文版）

基　　金：supported by Ministry of Science and Technology of China(Grant Nos.2009CB930001 and 2011CB933201);Chinese Academy ofSciences(Grant No.KJCX2-YW-M15);the National Natural ScienceFoundation of China(Grant Nos.20890020,90813032,21025520 and 51073045)

摘　　要：Different cell types make up tissues and organs hierarchically and communicate within a complex, three-dimensional (3D) en- vironment. The in vitro recapitulation of tissue-like structures is meaningful, not only for fundamental cell biology research, but also for tissue engineering (TE). Currently, TE research adopts either the top-down or bottom-up approach. The top-down approach involves defining the macroscopic tissue features using biomaterial scaffolds and seeding cells into these scaffolds. Conversely, the bottom-up approach aims at crafting small tissue building blocks with precision-engineered structural and functional microscale features, using physical and/or chemical approaches. The bottom-up strategy takes advantage of the repeating structural and functional units that facilitate cell-cell interactions and cultures multiple cells together as a functional unit of tissue. In this review, we focus on currently available microscale methods that can control mammalian cells to assemble into 3D tissue-like structures.Different cell types make up tissues and organs hierarchically and communicate within a complex, three-dimensional （3D） en- vironment. The in vitro recapitulation of tissue-like structures is meaningful, not only for fundamental cell biology research, but also for tissue engineering （TE）. Currently, TE research adopts either the top-down or bottom-up approach. The top-down approach involves defining the macroscopic tissue features using biomaterial scaffolds and seeding cells into these scaffolds. Conversely, the bottom-up approach aims at crafting small tissue building blocks with precision-engineered structural and functional microscale features, using physical and/or chemical approaches. The bottom-up strategy takes advantage of the re- peating structural and functional units that facilitate cell-cell interactions and cultures multiple cells together as a functional unit of tissue. In this review, we focus on currently available microscale methods that can control mammalian cells to assemble into 3D tissue-like structures.

关 键 词：microscale cell assembly tissue-like structures tissue engineering 

分 类 号：Q78[生物学—分子生物学] TK124[动力工程及工程热物理—工程热物理]