Effects of elevated CO_2 on the nutrient compositions and enzymes activities of Nilaparvata lugens nymphs fed on rice plants  

作　　者：ZENG YunYun HUANG WenKun SU Li WU Gang ZHUANG Jing ZHAO WanYun HUA HongXia LI JunSheng XIAO NengWen XIONG-YanFei 

机构地区：[1]'Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]State Key Laboratory for Biology of Plant Diseases and lnsect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China [3]Department of Plant Protection,Agricultural College,Guangxi University,Nanning 530005,China [4]State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012 China [5]Department of Biology,College of Science,Wuhan University of Technology,Hubei 430070,China

出　　处：《Science China(Life Sciences)》2012年第10期920-926,共7页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.31071691 and 31171846);the Major Projects of Cultivated Varieties of Genetically Modified Organisms(Grant No.2011ZX08012-005);the Self-determined and Innovative Research Funds of Wuhan University of Technology(Grant No.2011-1a-037),China

摘　　要：Elevated CO 2 may reduce the tolerance of Nilaparvata lugen (N. lugens) to adverse environmental factors through the biological and physiological degeneration of N. lugens. In an artificial climate box, under 375 and 750 μL L 1 CO 2 levels, the rice stems nutrient content, the nutrient content and enzyme activities of N. lugens nymph fed on rice seedlings exposed to ambient and elevated CO 2 were studied. The results showed that rice stems had significantly higher protein and total amino acid levels under ambient than elevated CO 2 levels. Nymphs had significantly higher protein levels in the ambient CO 2 treatment, while their glucose levels were significantly lower under ambient CO 2 conditions. Significantly higher trypsin activity was observed in nymphs grown in elevated CO 2 . Significantly lower activities of the protective enzymes total superoxide dismutase and catalase were observed in the nymphs under ambient CO 2 . Meanwhile, the activity of the detoxification enzyme glutathione S-transferase was significantly higher in the ambient CO 2 treatment. Measuring how energy and resources were allocated to enzymes in N. lugens nymphs under elevated CO 2 conditions can provide a more meaningful evaluation of their metabolic tolerances to adverse climatic conditions.Elevated CO2 may reduce the tolerance of Nilaparvata lugen （N. lugens） to adverse environmental factors through the biologi- cal and physiological degeneration of N. lugens. In an artificial climate box, under 375 and 750 μL L-1 CO2 levels, the rice stems nutrient content, the nutrient content and enzyme activities of N. lugens nymph fed on rice seedlings exposed to ambient and elevated CO2 were studied. The results showed that rice stems had significantly higher protein and total amino acid levels under ambient than elevated CO2 levels. Nymphs had significantly higher protein levels in the ambient CO2 treatment, while their glucose levels were significantly lower under ambient CO2 conditions. Significantly higher trypsin activity was observed in nymphs grown in elevated CO2. Significantly lower activities of the protective enzymes total superoxide dismutase and cat- alase were observed in the nymphs under ambient CO2. Meanwhile, the activity of the detoxification enzyme glutathione S-transferase was significantly higher in the ambient CO2 treatment. Measuring how energy and resources were allocated to enzymes in N. lugens nymphs under elevated CO2 conditions can provide a more meaningful evaluation of their metabolic tol- erances to adverse climatic conditions.

关 键 词：Nilaparvata lugens elevated CO2 rice plants total superoxide dismutase detoxification enzyme glutathioneS-transferase 

分 类 号：S718.512[农业科学—林学] S511.01