两个链霉菌复制子探针载体的构建  

CONSTRUCTION OF TWO REPLICATION PROBE VECTORS FOR STREPTOMYCES

作　　者：邓子新[1] T.Kieser D.A.Hopwood 

机构地区：[1]华中农业大学土化系农抗研究室 [2]英国约翰.英勒斯研究所

出　　处：《生物工程学报》1990年第2期157-161,共5页Chinese Journal of Biotechnology

摘　　要：因链霉菌质粒的大小、拷贝数各异,并不是所有的质粒都易于分离和纯化,尤其是低考贝的大质粒,质粒的分离提取往往耗资费力。在这种情况下。Incorporation of tsr gene from Streptomyces aureus and vph gene from Streptomyces vinaceus into pUC7-based vectors, two replication probe vectors for Streptomyces, pIJ 2703 and pIJ 2702, have been constructed. Both vectors carry the same amp gene from same origin for selection in Escherichia coli but carry either tsr or vph gene for selection in Streptomyces. tsr and vph genes can only be expressed in Streptomyces with the help of cloned Streptomyces replicon. Using neo gene as indictor, two vectors have properties of promoter-probe vectors.

关 键 词：链霉菌 复制子 探针载体 制备 

分 类 号：Q939.130.6[生物学—微生物学] Q81