钩藤碱微透析探针回收率测定及影响因素研究  

Recovery and its influencing factors of rhynchophylline microdialysis probe

作　　者：马博[1,2] 孙桂波[1] 李明[1,3] 孙晓波[1] 

机构地区：[1]中国医学科学院药用植物研究所,北京100193 [2]黑龙江中医药大学,哈尔滨150040 [3]温州医学院,温州325035

出　　处：《中国新药杂志》2012年第20期2371-2375,共5页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-003;2009ZX09502-015);国家科技部科研院所技术开发研究专项(2008EG150298);高等学校博士学科点专项科研基金(200800231106);中国博士后科学基金面上项目(2012M510360)

摘　　要：目的:考察体内外微透析探针回收率的稳定性和重现性,为进一步的体内微透析试验提供依据。方法:采用增量法和减量法考察浓度、流速对探针回收率的影响。结果:微透析探针回收率受流速影响较大,基本不受浓度影响,且体内回收率稳定性良好。结论:微透析技术可用于钩藤碱的体内药代动力学研究,反透析法可作为体内研究钩藤碱探针回收率的测定方法。Objective： To study the recovery of rhynchophylline microdialysis probe in vivo and in vitro by external standard method and provide basis for further in vivo microdialysis experiments. Methods： The concentra tion and flow rate on the recovery of the probe was determined by incremental and reduction methods. Results： The flow rate had a significant impact on the microdialysis probe recovery, and the concentration had little influence on the recovery. The in vivo probe recovery showed a good stability. Conclusion： Microdialysis can be used in rhyncho phylline pharmacokinetic studies, and retrodialysis method can be used in probe recovery of rhynchophylline in vivo.

关 键 词：微透析 钩藤碱 探针回收率 

分 类 号：R969.11[医药卫生—药理学]