应用PCR-DGGE法评价石斛多糖对肠道微生态失调的调节作用  

Regulatory effects of dendrobium polysaccharides on intestinal dysbiosis: an evaluation by PCR-DGGE

作　　者：郭建壮[1] 成正祥[1] 祁冬冬[1] 马淑霞[1] 杜宁 赵宏博[3] 杨景云[1] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007 [2]青岛妇幼儿童医院,山东青岛266000 [3]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《中国微生态学杂志》2012年第10期873-875,共3页Chinese Journal of Microecology

基　　金：国家"973"课题(2007CB513008):微生态调节剂防治感染的基础研究

摘　　要：目的从微生态学角度研究石斛多糖治疗肠道微生态失调小鼠的作用及初步机制。方法盐酸林可霉素灌胃制备肠道菌群失调小鼠模型,应用石斛多糖进行治疗,同时设正常对照组、丽珠肠乐组、阴性对照组,给药7 d后处死小鼠,应用PCR-DGGE法检测肠道菌群丰富度、血清IL-2。结果应用盐酸林可霉素灌胃3 d后,小鼠肠道菌群丰富度、血清IL-2降低,持续治疗7 d后,石斛多糖治疗小鼠肠道菌群丰富度、血清IL-2增高。结论石斛多糖具有扶植肠道正常菌群生长,调整菌群失调,提高机体免疫力的作用。Objective To explore the role and preliminary mechanism of Dendrobium Polysaccharides in treating mice intestinal microflora imbalance from a micro-ecological perspective.Methods Lincomycin hydrochloride was orally administered to induce intestinal microflora imbalance in the mice,after which the mice were treated with Dendrobium Polysaccharides.At the same time,the mice were randomly divided into normal control group,Oral Live Bifodobacterium preparation group and negative control group.After 7 days of treatment with various medicines,the mice were sacrificed and the PCR-DGGE and ELISA were used to detect intestinal flora richness and serum IL-2 respectively.Results After 3 days of oral administration of Lincomycin hydrochloride,there was a decrease in the intestinal flora richness and serum IL-2 of the mice.However,after 7 days of continuous treatment with Dendrobium Polysaccharides,an increase in the intestinal flora richness and serum IL-2 were observed.Conclusion Dendrobium Polysaccharide fosters the growth of normal intestinal flora,has a regulatory effect on intestinal flora,and improves immunity.

关 键 词：石斛多糖 肠道菌群 微生态学 PCR-DGGE 

分 类 号：Q949.718.43[生物学—植物学]