产萱草根素内生真菌的分离与鉴定  

Isolation and Identification of Hemerocallin-Producing Fungal Endophyte

作　　者：梁洁[1] 杨凯[1] 李勤凡[1] 姜振国[1,2] 孔祥雅[1] 杨国栋[1] 金意敏[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]西安市长安区动物疾病预防控制中心,陕西长安710100

出　　处：《草地学报》2012年第5期935-939,共5页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(30871901);陕西省科技攻关项目(2008k02-06-2)资助

摘　　要：为探讨萱草(Hemerocallis)根中是否存在产生萱草根素的内生真菌,从北萱草(H.esculenta)根中分离得到4株内生真菌,在马铃薯(Solanum tuberosum)葡萄糖琼脂培养基(PDA)上培养20d后收集菌丝,应用薄层色谱法和紫外分光光度法分别检测菌丝提取液中的萱草根素,筛选可产生萱草根素的内生真菌。结果表明:菌株XC-1A为产萱草根素内生真菌,含量达359.88μg·g-1,根据形态学观察和5.8S rDNA-ITS序列分析结果,确定XC-1A为Zalerion varium。北萱草中存在可产生萱草根素的内生真菌。The study was to explore whether the hemerocallin-secret fungal endophyte exist in roots of Hemerocallis.Four strains of endophytic fungi were isolated and obtained from the H.esculenta.Four isolates were detected by thin-layer chromatography and UV spectrophotometry after culture in potato dextrose agar（PDA） for 20 days.Results showed that XC-1A,a strain of endophytic fungus producing hemerocallin was isolated and obtained from H.esculenta,and hemerocallin yield is 359.88 μg·g-1.Morphological evidence and rDNA-ITS sequence analsis indicated that the endophyte was closely related to Zalerion varium.The hemerocallin-producing fungal endophytes exist in the root of H.esculenta.

关 键 词：北萱草 萱草根素 内生真菌 

分 类 号：Q948.122.3[生物学—植物学] Q93-331