穿膜肽提高纳米银穿膜活性的研究  

The Penetrating Activity of Nano-silver Mediated by Cell Penetrating Peptide

作　　者：刘金华[1,2] 郭倩倩[1,2] 华海婴[1] 袁波[3] 赵永星[1] 黄永焯[2] 

机构地区：[1]郑州大学,郑州450001 [2]中科院上海药物所,上海201203 [3]山东中医药大学附属医院,济南250000

出　　处：《中国现代应用药学》2012年第10期865-868,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金(91029743;81172996)

摘　　要：目的合成纳米银,在其表面修饰穿膜肽(TAT),并检测修饰后纳米银粒对人乳腺癌耐阿霉素细胞(MCF-7/ADR)的穿膜活性。方法通过化学还原法制备纳米银(AgNP),并通过Ag-S共价键与TAT连接修饰AgNP。通过粒度仪、透射电镜、激光共聚焦显微镜、流式细胞仪以及二喹林甲酸法等仪器及方法对其表征、共价连接及TAT的介导活性进行测定。结果成功制备了10 nm以下的AgNP,且修饰TAT后的AgNP(AgNP-TAT)表现出了比AgNP更强的穿膜活性。结论 TAT修饰AgNP后能显著提高其穿膜活性。OBJECTIVE To assess the intracellular delivery efficiency of silver nanoparticles surface-modified by cell penetrating peptides（TAT） in human breast cancer drug doxorubicin-resistant MCF-7/ADR cells. METHODS Silver nanoparticle（AgNP） was prepared by chemical reduction and was modified with TAT by Ag-S covalent bond. The TAT-AgNP was characterized using particle analyzer, TEM, laser confocal microsopy, and TGA, and TAT-mediated cellular uptake were measured using FACS. RESULTS The AgNP with diameter below 10 nm was successfully synthesized. The cell penetrating activity of TAT-modified AgNP （AgNP-TAT） was higher than that of AgNP. CONCLUSION The cell penetrating activity of AgNP was significantly improved by modification with TAT.

关 键 词：纳米银 穿膜肽 MCF-7/ADR 细胞摄取 

分 类 号：R966[医药卫生—药理学]