近红外漫反射光谱法快速测定盾叶薯蓣药材醇浸出物的含量  

Quantitative Analysis on Extracts by Ethanol from Dioscorea Zingiberensis C.H. Wright by Near-infrared Diffuse Reflection Spectroscopy

作　　者：谢彩侠[1] 左春芳[1] 雷敬卫[1] 白雁[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国现代应用药学》2012年第10期931-935,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：河南省高等学校青年骨干教师资助计划(2010GGJS-134);河南省教育厅自然科学研究项目(2011A360021)

摘　　要：目的应用近红外光谱(NIR)技术和化学计量学分析软件,对盾叶薯蓣药材中醇浸出物的含量进行快速测定。方法应用偏最小二乘法(PLS)对盾叶薯蓣醇浸出物的结果与NIR建立定量校正模型。结果建立的模型内部交叉验证决定系数(R2)为0.991 09,内部交叉验证均方差(RMSECV)为0.010 13,预测均方差(RMSEP)为0.004 59。结论近红外漫反射光谱技术的运用具有快速方便、结果准确的特点,该方法可应用于盾叶薯蓣药材醇浸物的快速检测,对于其他的中药材指标成分测定也有一定的参考价值。OBJECTIVE To determine the content of extracts from Dioscorea zingiberensis C.H.Wright by near-infrared spectroscopy combined with chemometrics method. METHODS Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the extracts values. RESULTS The correlation coefficients （R2）, The root-mean-square error of cross-validation （RMSECV） and the root-mean-square error ofprediction（RMSEP） of the quantitative analysis model for Dioscorea zingiberensis C.H.Wright were 0.991 09, 0.010 13 and 0.004 59. CONCLUSION NIRS technique is rapid and correct, which can be used in the determination of extracts from Dioscorea zingiberensis C.H.Wright. It also can be applied in other Chinese herbs.

关 键 词：近红外漫反射光谱法 偏最小二乘法 定量分析 盾叶薯蓣 浸出物 

分 类 号：R917.101[医药卫生—药物分析学]