灰斑古毒蛾口腔反吐物诱导沙冬青细胞Ca^(2+)内流及H_2O_2积累  

Regurgitant from Orgyia ericae Germar induces calcium influx and accumulation of hydrogen peroxide in Ammopiptanthus mongolicus(Maxim.ex Kom.) Cheng f.cells

作　　者：高海波[1] 张淑静[2] 沈应柏[2] 

机构地区：[1]临沂大学生命科学学院,临沂276005 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《生态学报》2012年第20期6520-6526,共7页Acta Ecologica Sinica

基　　金：国家自然科学资助项目(30871727);高等学校博士学科点专项科研基金(200900141100/4)

摘　　要：植物对昆虫取食活动进行成功防御的关键,取决于对昆虫口腔反吐物的激发子的快速识别。实验利用无损伤微测系统及激光共聚焦显微镜,研究了沙冬青细胞经灰斑古毒蛾口腔反吐物诱导后Ca2+流及H2O2的变化。结果发现:灰斑古毒蛾口腔反吐物诱导沙冬青细胞Ca2+内流及H2O2的积累,表明Ca2+内流及H2O2的积累是沙冬青细胞对口腔反吐物产生应答的早期响应事件;Ca2+钙通道阻断剂仅部分抑制Ca2+内流,说明Ca2+内流除经过质膜上的Ca2+通道进入细胞外,尚存在其他的内流途径;灰斑古毒蛾口腔反吐物中的某些成分与沙冬青细胞的质膜结合后,诱导质膜上形成允许Ca2+通过的孔道,而GdCl3不能抑制这类孔道的活性。胞外Ca2+螯合剂EGTA完全抑制H2O2的积累,GdCl3预处理仅部分抑制了H2O2的积累,说明灰斑古毒蛾诱导的沙冬青细胞内H2O2的积累依赖于Ca2+内流;抑制剂实验表明,H2O2的积累主要来源于质膜上NADPH氧化酶的作用。The battle between plants and phytophagous insects has lasted for hundreds of millions of years.Over time,plants have evolved sophisticated defence systems to counteract attacks from insects.After being attacked by herbivores,plants quickly generate herbivory-specific signals,and,through complicated networks,these signals then trigger large-scale biochemical and physiological changes.The success of plants in withstanding herbivore attacks depends on their ability to quickly recognize elicitors in insect regurgitant.When herbivorous insects feed on plants,their regurgitant inevitably comes in contact with the wounded plant tissue.Thereby,the insects provide chemical signals that might be involved in the interaction between the attacking insect and the defending plant.In general,the earliest detectable signalling events in plants defence responses include transmembrane ion fluxes and production of reactive oxygen species.In plant cells,the calcium ion is a ubiquitous intracellular second messenger involved in numerous signalling pathways.It is widely acknowledged that calcium flux across cellular membranes plays a key role in triggering and mediating defence mechanisms.Hydrogen peroxide is a common component of the defence responses of plants against herbivore attacks.The non-invasive microelectrode ion flux measurement technique,which can monitor ion/molecule-specific activities non-disruptively,has become a popular tool for studying adaptive responses of plant cells and tissues to a large number of abiotic stresses.Using a non-invasive micro-test system and a confocal laser scanning microscope,Ca2＋ influx and variation in hydrogen peroxide production induced by the regurgitant of Orgyia ericae Germar were investigated in Ammopiptanthus mongolicus（Maxim.ex Kom.） Cheng f.cells.Experiments were conducted in the Key Laboratory of Forest Silviculture of the State Forestry Administration from June 2010 to April 2011.The results showed that Ca2＋ influx and hydrogen peroxide accumulation were induced by the regur

关 键 词：沙冬青 非损伤微测系统 灰斑古毒蛾 Ca2+流 H2O2 

分 类 号：S433[农业科学—农业昆虫与害虫防治]