西南产玄参药材的HPLC指纹图谱研究  

HPLC Fingerprint of Scrophulariae Radix from Southwest China

作　　者：周雯[1] 蒋林[1] 王晓娟[1] 陶红[1] 郑锡康[1] 胡继藤[1] 

机构地区：[1]中山大学药学院生药与天然药物化学实验室,广东广州510006

出　　处：《中药材》2012年第8期1230-1233,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:建立西南不同产区玄参药材及市售玄参饮片甲醇提取物的HPLC指纹图谱,为其质量控制提供依据。方法:用HPLC分析测定重庆南川、酉阳及贵州道真产3批玄参药材及8批市售玄参饮片,色谱条件:C18柱,乙腈-0.4%冰醋酸为流动相进行梯度洗脱,检测波长260 nm,流速1.0 mL/min,检测时间55 min。结果:以哈巴俄苷及肉桂酸为指标性成分,建立了玄参药材及饮片的HPLC指纹图谱共有模式图,对不同产地的样品进行相似度比较,相似度大于0.901。结论:该指纹图谱检测方法快速、简便,有助于全面评价玄参药材的品质,也为玄参规范化种植提供理论依据。Objective： To establish HPLC fingerprints of Scrophulariae Radix methanol extract from both the different southwest origin and market,provide evidence for its quality control.Methods： 11 batches of samples analysis was performed on C18 column with gradient mobile phase of acetonitrile-0.4% acetic acid,the monitoring wavelength was at 260 nm with the flow rate of 1.0 mL/min,detective course was finished in 55 min.Results： Established the HPLC fingerprint common patterns by using harpagoside and cinnamic acid as the index components,and similarity with different origin rude drugs and pieces was compared.The similarity was above 0.901.Conclusion： This method contributes to the fast comprehensive evaluation of material quality and can be used specifically for standardized planting and quality control of Scrophulariae Radix.

关 键 词：玄参 哈巴俄苷 肉桂酸 高效液相色谱法 指纹图谱 

分 类 号：R282.5[医药卫生—中药学]