硫化氢对脂多糖诱导急性肺损伤的保护作用  

Protective role of hydrogen sulfide on acute lung injury induced by lipopolysaccharide

作　　者：黄继义[1] 叶先龙[1] 刘才文[1] 洪朝基[1] 周荣[1] 

机构地区：[1]厦门大学附属第一医院翔安分院急诊科,福建厦门361101

出　　处：《中国急救医学》2012年第10期907-909,I0001,共4页Chinese Journal of Critical Care Medicine

基　　金：厦门市翔安区科技局资助项目（2010XA0811）

摘　　要：目的探讨硫化氢对急性肺损伤（acutelunginjury，ALI）的保护作用及其机制。方法雄性SD大鼠共48只，随机分为脂多糖组（n=24）和脂多糖＋NariS组（n=24）。3h后检测肺组织髓过氧化物酶（MPO）活性、丙二醛（MDA）含量、核转录因子-KB（NF—KB）活性、Toll样受体（Tolllikereceptor，TLR）4mRNA、巨噬细胞移动抑制因子（macrophagemigrationinhibitoryfactor，MIF）mRNA、肺湿质量／干质量（W／D）、肺组织的病理变化和外周血中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）的含量。结果脂多糖＋NariS组肺损伤显著改善。与脂多糖组比较，脂多糖＋NaHS组肺组织TLR4和MIFmRNA表达降低，MPO活性、MDA含量、肺W／D下降，血浆TNF-α、IL-6含量降低。结论硫化氢可通过抑制TLR4和MIF的表达减轻ALI的肺部炎性反应。Objective To study protective effect of hydrogen sulfide （H2S） against acute lung injury and its underlying mechanisms. Methods Fourty - eight Sprague - Dawley rats were randomly divided into lipopolysaccharide （LPS） group and LPS ＋ NariS group, with 24 rats in each. After 3 hours, myeloperoxidase （MPO） activity, malondialdehyde （MDA）, nuclear factor - KB （ NF - KB） activity, toll- like receptors （TLR） 4 mRNA, macrophage migration inhibitory factor （MIF） mRNA, the wet - to - dry weight （W/D） ratio, and histological changes of the lung tissue were measured. Blood sample was collected to analysis tumor necrosis factor -α （ TNF -α） and interleukin - 6 （ IL - 6）. Results LPS ＋ NariS significantly improved the lipopolysaccharide - induced histological changes. TLR 4 mRNA, MIF mRNA, MPO, NF -KB activity, MDA, and W/D ratio of lung tissue were significantly lower in the LPS ＋ Naris group than those in the LPS group （P 〈 0.05）. Serum level of TNF -α and IL - 6 were also significantly lower in the LPS ＋ Naris group （P 〈 0. 05 ）. Conclusion These results suggest that H2S protects against acute lung injury by inhibiting TLR4 and MIF.

关 键 词：硫化氢 急性肺损伤(ALI) Toll样受体4(TLR4) 巨噬细胞移动抑制因子(MIF) 

分 类 号：R543.2[医药卫生—心血管疾病]