碘过量与富碘中药对碘缺乏大鼠甲状腺内氧化应激的比较研究  

Comparison of oxidative stresses in thyroid gland between excessive iodine intake and iodine-rich herbs in rats with iodine deficiency

作　　者：高天舒[1] 齐腾澈[1] 

机构地区：[1]辽宁中医药大学附属医院内分泌科,沈阳110032

出　　处：《中华内分泌代谢杂志》2012年第10期855-858,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：高等学校博士学科点专项科研基金（20092133110004）.辽宁省教育厅（2009A492）;辽宁省教育厅优秀人才计划（LR201026）

摘　　要：选4周龄Wistar大鼠120只，制成碘缺乏甲状腺肿模型后分成4组：正常对照组（NC）、模型对照组（MC）、碘过量组（IE）和富碘中药组（HIE）。在造模成功0周和8周后处死大鼠。生化方法测定大鼠血清谷胱甘肽过氧化物酶（GSH—Px）、黄嘌呤氧化酶（XOD）和超氧化物歧化酶（SOD）的活力，及丙二醛（MDA）、过氧化氢（H2O2）的含量。免疫组化方法检测甲状腺中4-羟基壬烯醛（4-HNE）的表达。Western印迹及免疫组化法分析过氧化物氧化还原酶5（PRDX5）在大鼠甲状腺的表达。治疗8周后，SOD活力IE组较HIE组显著降低（P〈0．05）。GSH—Px活力IE组和HIE组均较MC组显著降低（均P〈0．01），H2O2含量IE组较NC组显著增高（P〈0．05）。MDA含量IE组较HIE组显著增高（P〈0．05）。XOD活力IE组和HIE组较NC组显著增高（均P〈0．01）。甲状腺4-HNE表达，IE组较HIE组比较显著增高（P〈0．05）。甲状腺PRDX5表达免疫组化结果和Westernblot结果共同显示HIE组较其他各组均显著增高（均P〈0．05）。与碘过量比较。富碘中药对碘缺乏机体甲状腺细胞损伤轻，甲状腺肿恢复完全，这可能与富碘中药在补充碘的同时又增强了抗氧化能力有关。One-hundred and twenty 4-week-old Wistar rats were selected. After making the model of goiter by iodine deficiency, the rats were divided into 4 groups ： normal control group （ NC）, model control group （ MC）, excessive iodine group（IE） and herbs rich in iodine group（ HIE ）. The rats were killed 0 week and 8 weeks after different treatments. The activity of rat serum glutathione peroxidase （ GSH-Px）, xanthineoxidase （ XOD ）, and superoxide dismutase （ SOD）, as well as the levels of malondialdehyde （MDA） and hydrogen peroxide （ H2 02 ） , were measured by biochemical methods. The expression of 4-hydroxynonenal （4-HNE） in the rat thyroid was detected by immunohistochemistry method. The expression of peroxiredoxin5 （ PRDX5 ） in the rat thyroid was detected by the Western blotting and immunohistochemistry. After 8 weeks of different treatments, the SOD activities in IE group decreased significantly compared with HIE group（P〈0.05 ）. While the GSH-Px activities in IE group and HIE group decreased significantly compared with MC group （ both P 〈 0. 01 ） , the values of H2 02 in IE group increased significantly compared with NC group （ P〈0.05 ）. The values of MDA in IE group increased markedly COnlpared with HIE group （ P〈0.05 ）. The XOD activities in IE group and HIE group were significantly higher than NC group. （ both P〈0.01 ）. The expression of thyroid 4-HNE in IE group was significantly higher than HIE group （ P〈0.05 ）. The expression of thyroid PRDX5 in HIE group was markedly higher than that in the other groups, as shown by both immunohistochemistry and Western blotting methods （ all P〈0.05 ）. Compared with excessive iodine intake, herbs rich in iodine may cause less damage to thyroid follicular cells and make iodine deficiency induced goiter recover more completely, suggesting that this improved result may be related to the enhanced antioxidant capacity, owing to administering iodine-rich herbs.

关 键 词：甲状腺肿 氧化应激 碘过量 富碘中药 

分 类 号：R581[医药卫生—内分泌]