两个育性相关基因在几类小麦雄性不育材料中的表达研究  

Expression Analysis of Two Genes Related to Male Fertility in A Few Types of Male Sterile Wheat

作　　者：刘菲[1] 马晓飞[1] 李艳飞[1] 王爱芳[1] 靳凤[1] 马翎健[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100

出　　处：《西北植物学报》2012年第9期1731-1735,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家科技支撑计划(2011BAD35B03);西北农林科技大学基础业务费专项基金(ZD2012001)

摘　　要：以1B/1R类K型不育系K3314A及其保持系3314B,非1B/1R类K型不育系732A及其保持系732B,YS温敏雄性不育小麦A3017为材料,提取不同温度处理下各时期小穗的总RNA进行反转录,对AY914051和AY660990两个目标基因进行半定量PCR和电泳分析,测定其在小麦中的表达变化趋势,以分析其与这几种不育系的育性相关关系,探讨这几种不育小麦败育的关键发育时期。结果表明,除AY914051基因在2种保持系3314B和732B中表达量变化不大之外,其它均有明显差异;2个目标基因与这几种雄性不育系的败育有关,但与育性相关程度不同,不育系两个目的基因的表达受温度变化影响程度明显大于保持系。由实验结果推测,1B/1R类K型不育系、非1B/1R类K型不育系和YS温敏雄性不育系的败育关键时期为单核期或单核期至二核期之间;温度差异可能会导致YS温敏雄性不育系的育性相关基因表达时期错位,错位后的表达差异积累可能最终导致其败育。The various tendency of two target genes’ expression in several male sterility wheats was measured to determine whether these genes related to these types of wheat male sterile lines’ fertility,and to analyze the key period of abortion in these several male sterility wheats.The total RNA was extracted at various periods of spikelet development at different temperature treatments,then was reverse-transcripted to cDNA with experiment materials,1B/1R K-type wheat male sterile line K3314A and its maintainer line 3314B,non-1B/1R K-type wheat male sterile line 732A and its maintainer line 732B and YS type male sterile line A3017.The expression patterns of 3314B and 732B related to male fertility were analyzed with semi-quantitative PCR.The results showed that there were significant differences in the expression of those two genes among those materials and temperature treatments,except that gene AY914051 in two maintainer lines 3314B and 732B.The expression of the two genes was related to the abortion of pollen development in these male sterile lines with different effects.The expression of the two genes in male sterile lines was affected more significantly by the changing temperature than that in maintainer lines.It can be guessed the key development period of pollen abortion in 1B/1R K-type wheat male sterile line,non-1B/1R K-type wheat male sterile line and YS type male sterile line were the mononuclear period or that between mononuclear period and dinuclear period.The lower temperature might change the expression patterns of genes related to male fertility at different stages,and the accumulation of expression differences likely to eventually lead to the abortion of pollen development.

关 键 词：雄性不育小麦 人工控温 败育关键时期 半定量分析 

分 类 号：Q343.3[生物学—遗传学]