张杂谷苹果酸脱氢酶和过氧化物酶同工酶研究  

Studies on Isozymes of MDH and POD of Hybrid Millet Zhang in Different Growing Stages

作　　者：郭秀林 刘子会[1,2] 王云 张红梅[1,2] 张艳敏 李慧聪[1,2] 赵治海 

机构地区：[1]河北省植物转基因中心重点实验室 [2]河北省农林科学院遗传生理研究所,石家庄050051 [3]河北省张家口市农科院,河北张家口076650

出　　处：《西北植物学报》2012年第9期1768-1773,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家科技支撑计划(2011BAD06B00);河北省应用基础研究计划重点基础研究(11965517D)

摘　　要：以张杂谷‘3号’、‘5号’、‘6号’及其亲本为材料,通过聚丙烯酰胺垂直板凝胶电泳技术研究不同材料不同生育期苹果酸脱氢酶和过氧化物酶同工酶酶谱特性,同时对总酶活进行了测定,探讨谷子杂种优势的形成机理。结果表明:(1)张杂谷及其亲本叶片苹果酸脱氢酶共有5条酶带,有共同酶带和偏母本型酶带;过氧化物酶同工酶共有14条,属于共同酶带;从负极到正极苹果酸脱氢酶同工酶Rf分别为0.611、0.626、0.642、0.684和0.716,而过氧化物酶同工酶Rf分别为0.08、0.21、0.24、0.30、0.36、0.44、0.49、0.58、0.66、0.69、0.72、0.75、0.85和0.89。(2)杂种苹果酸脱氢酶同工酶和母本酶谱同型,过氧化物酶同工酶酶谱和父母本一致;供试品种苹果酸脱氢酶酶谱相对简单,酶带集中,而过氧化物酶同工酶酶带数量、活性和宽度差异性较大,酶谱比较复杂。(3)所有品种苹果酸脱氢酶总活性在抽穗期最高,过氧化物酶总活性在灌浆初期最高。杂种苹果酸脱氢酶活性在苗期和抽穗期表现出不同程度的超亲优势,而过氧化物酶在抽穗和灌浆初期均表现出不同程度的超亲优势,这可能与杂种优势有关。Two isozymes of malate dehydrogenase（MDH） and peroxidase（POD） were performed by PAGE,and their activities were measured to study the formatting mechanism of heterosis of foxtail millet using hybrid millet ’Zhang No.3,5,6’ and their parents,respectively.The results indicated that：（1）There were 5 bands of MDH isozyme which belong to homotype and tend-female type in leaf of hybrid millet Zhang and their parents,and 14 bands of POD isozyme which belong to homotype.（2）The isozyme patterns of MDH between hybrid millet Zhang were same as that of their female parent,and that of POD was same as their parents.The tested individuals and parents differed in number,Rf,activity or width of the POD bands,the zymogram was complicated,but it was simple in MDH.（3）The MDA activities of all varieties were the highest in heading stage and there was heterosis of MDH activity in hybrid millet Zhang from seedling to heading stage,and the POD activity was the highest in early grain-filling stage and there was heterosis from heading to early grain-filling stage too,showing a correlation exists between the isozyme patterns both MDH and POD with the formation of heterosis in hybrid millet Zhang.

关 键 词：张杂谷 苹果酸脱氢酶 过氧化物酶 同工酶 杂种优势 

分 类 号：Q346.5[生物学—遗传学]