藏绵羊毛囊组织中microRNA-31的表达量变化  

Expression Variation of microRNA-31 in Wool Follicle of Tibetan Sheep(Ovis aries)

作　　者：唐晓惠[1,2] 柳广斌[1] 杜小勇[1] 曹建华[1] 李新云[1] 罗章[2] 余梅[1] 赵书红[1] 

机构地区：[1]华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉430070 [2]西藏农牧学院动科学院,林芝860000

出　　处：《农业生物技术学报》2012年第10期1178-1183,共6页Journal of Agricultural Biotechnology

基　　金：国家自然基金地区项目(No.30960163);转基因生物新品种培育重大专项(No.2008ZX08009-004)

摘　　要：microRNA是一种小分子非编码RNA,参与生物生命过程的多个环节,其对细胞增值,细胞分化,组织发育等都有重要的调控作用。为了研究microRNA-31在绵羊毛囊发育中的作用,本研究以藏绵羊(Ovisaries)毛囊组织为研究材料,利用荧光定量PCR(qPCR)方法检测了microRNA-31在毛囊3个不同发育时期中的表达变化情况,并对绵羊microRNA-31进行了基因组定位及前体预测,预测了2个可能的靶基因Keratin17(KRT17)和distal-less homebox3(DLX3),并进行了qPCR验证。结果发现microRNA-31的表达量从毛囊的生长期到休止期呈下降趋势,其序列定位在绵羊2号染色体上。研究结果提示,microRNA-31在绵羊毛囊发育中可能发挥着重要作用,KRT17可能是受其调控靶基因之一。MicroRNA is a class of noncoding small RNA. It is involed in the regulation of many parts of life processes, and plays an important role in cell proliferation, differentiation and tissue development. In order to study the roles ofmicroRNA-31 in wool follicle development of sheep, we collected wool follicle samples from Tibetan sheep （Ovis aries） during three different phases including anagen, catagen and telogen. The results showed that there was a significance change of the expression of microRNA-31 during wool follicle development by quantitative Real-time （qPCR）. Resuks showed that the expression of microRNA-31 was decreased from anagen to telogen and the expression in anagen was about 1.5 fold than catagen （P〈0.01）. In addition, we predicted the precursor sequence of sheep microRNA-31 by miRdeep2 software and found that it was located on the negative strand of sheep chromosome 2 from 89 779 078 to 89 779 137 nucleotides （OAR2.0 version）. Furthermore, we predicted the target genes by RNA22 software. We found that two candidate genes, Keratin 17 （KR T17） and distal-less homebox3 （1）LX3）, existed potential binding sites of microRNA-31 in sheep. Then we detected the expression variation of KRT17 and DLX3 genes in anagen, catagen and telogen wool follicle samples by qPCR. As a result, the expression of KRT17 was increased from anagen to telogen. The level in telogen was almost 3 fold than anagen （P〈0.01）. It was a reverse pattern of the expression of microRNA-3 I. However, the expression of DLX3 in catagen was higher than the other two phases （catagen and telogen）（P〈0.01）. Conclusion, we suggested that microRNA-31 may play an impotent role during wool follicle development, and KR TI 7 probably is one of the target genes which are regulated by microRNA-31.

关 键 词：microRNA-31 毛囊 绵羊 

分 类 号：S826.1[农业科学—畜牧学]