1,25-二羟基维生素D_3对大鼠体重、血生化及CD4^+CD25^+Treg细胞的影响  

Effects of 1,25-(OH)_2D_3 on body weight,blood biochemistry and regulatory CD4^+ CD25^+ Treg cells of experimental rats

作　　者：祁晓平[1] 李培[1] 恽时峰[1] 黎介寿[1] 

机构地区：[1]南京大学医学院南京军区南京总医院全军普通外科研究所,江苏南京210002

出　　处：《中国新药与临床杂志》2012年第10期594-597,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：国家重点基础研究发展规划项目资助(2009CB522405)

摘　　要：目的了解1,25-二羟基维生素D_3[1,25-(OH)_2D_3]对大鼠体重、血生化及CD4^+CD25^+Treg细胞的影响。方法 40只Lewis大鼠在正常膳食标准钙含量的状态下,分为对照组和1,25-(OH)_2D_30.125μg、0.25μg、1μg 3个剂量组,每周一、三、五分别灌胃给予相应剂量1,25-(OH)_2D_3,给药2 wk,对照组给予等容积赋形剂,每周3次记录大鼠体重变化,d 15在麻醉状态下留取大鼠外周血和脾脏组织,采用全自动生化分析仪检测血生化,流武细胞仪检测CD4^+CD25^+Treg细胞数量,real-time PCR法检测脾脏Foxp3 mRNA表达水平。结果与对照组相比,1,25-(OH)_2D_31μg组大鼠体重增长减慢(P<0.05);1μg组和0.25μg组大鼠血钙升高,0.125μg组大鼠血钙正常;1,25-(OH)_2D_3各剂量组大鼠血脂水平均轻度降低(P<0.05),外周血及脾脏CD4^+CD25^+Treg细胞的数量和脾脏Foxp3 mRNA表达水平显著升高(P<0.05),但肝、肾功能无显著影响(P>0.05)。结论在正常膳食的情况下,间断给予1,25-(OH)_2D_30.125μg大鼠血钙正常、对CD4^+CD25^+Treg细胞的诱导作用明显,安全有效。AIM To investigate the effect of 1, 25-（OH） 2D3 on weight, blood biochemistry and CD4＋ CD25 ＋Treg cells in rats. METHODS Fourty male Lewis rats fed standard chow divided into 4 groups and treated with either 0.125 μg, 0.25 μg, 1 μg of 1, 25- （OH） 2D3 or vehicle on Monday, Wednesday and Friday respectively for 2 weeks. Body weight of these rats was recorded 3 times a week. On day 15, the rats were anesthetized by amiodarone, peripheral blood and spleen tissues were taken to count CD4＋ CD25＋ Treg cells by flow cytometry. Expression of Foxp3 mRNA in spleen was measured by real- time PCR method. The blood calcium, lipids, liver function and renal function were examined by automatic biochemistry analyzer. RESULTS Compared with the control group, the increasing of rat body weight were delayed in the 1 μg dose of 1, 25- （OH） 2D3 group （P 〈 0.05）. In addition, serum calcium of rats were increased in the 1 μg and 0.25 μg dose of 1, 25-（OH） 2D3 group （P 〈 0.05）, but not in 0.125 μg group （P 〉 0.05） . The levels of blood lipids were decreased, the number of CD4＋ CD25＋ Treg cells in peripheral blood and spleen were increase and the mRNA expression of Foxp3 in spleen were enhanced in all 1, 25-（OH） 2D3 groups （P 〈 0.05）. Moreover, liver and renal functions were no influences （P 〉 0.05） . CONCLUSION 1, 25- （OH） 2D3 with 0.125 μg interval treatment would be safe and effective with normal serum calcium and significant effects on CD4＋ CD25＋ Treg cells for rats fed standard chow.

关 键 词：1 25-二羟基维生素D_3 体重 钙 自身免疫疾病 CD4^+ CD25^+ Treg细胞 Foxp3 

分 类 号：R392.5[医药卫生—免疫学]