丙泊酚对大鼠脑发育期学习记忆功能和海马长时程增强的影响  

Effect of propofol on learning and memory function and long-term potentiation of hippocampus in developing brain of rats

作　　者：彭淑君[1] 陈萍[1] 高进[1] 向诗琪[1] 

机构地区：[1]重庆医科大学附属第一医院麻醉科,重庆400016

出　　处：《中国新药与临床杂志》2012年第10期602-606,共5页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的探讨丙泊酚对新生大鼠幼年期学习记忆功能和海马CA1区长时程增强(LTP)的影响。方法健康SD大鼠36只,日龄7d,随机分为3组(n=12):对照组(C组)、丙泊酚单次注射组(P1组)和丙泊酚多次注射组(P2组)。C组腹腔注射生理盐水7.5mL·kg^(-1)·d^(-1),连续7d;P1组腹腔注射生理盐水7.5mL·kg^(-1)·d^(-1),连续6d,d7注射丙泊酚75mg·kg^(-1);P2组腹腔注射丙泊酚75mg·kg^(-1)·d^(-1),连续7d。于d7注射完毕后15 min,各组抽取4只大鼠左心室穿刺抽取动脉血行血气分析和血糖检测,其余大鼠喂养至出生d 28进行Morris水迷宫实验,测试空间学习记忆功能。水迷宫实验结束后24 h,制备海马脑片,记录电刺激诱导的兴奋性突触后场电位(fEPSP)的变化和LTP诱导成功率。结果 3组血气分析指标和血糖均在正常范围内。Morris水迷宫实验中d3开始P2组的逃逸潜伏期较C组和P1组延长,寻台路径亦长于C组和P1组(P<0.05)。P2组探索时间较C组和P1组减少,穿越平台区的次数亦减少(P<0.05)。C组和P1组的空间学习记忆功能无显著差异(P>0.05)。条件刺激后P1组和P2组的fEPSP斜率均低于C组,且P2组低于P1组(P<0.05)。P2组LTP诱导成功率低于C组和P1组(P<0.05),C组和P1组间无显著差异(P>0.05)。结论多次注射丙泊酚可抑制大鼠离体海马脑片CA1区LTP的形成,导致新生大鼠幼年时期空间学习记忆功能减退。AIM To investigate the effect of propofol anesthesia on learning and memory function and long-term potentiation （LTP） in CA1 region of hippocampus when neonata rats grow to infancy. METHODS Thirty-six 7-day-old SD rats were randomly divided into 3 groups （12 in each）. The rats in the group C were intraperitoneally injected with normal saline （NS） 7.5 mL·kg^-1·d^-1 for 7 d. The rats in the group P1 were injected NS 7.5mL·kg^-1·d^-1 for 6 d and propofol 75 mg.kg^-1 （7.5 mL.kg^-1） on the d 7. The rats in the group P2 were injected propofol 75 mg-kg-1 ＂d-1 for 7 d. Fifteen minutes after the last intraperitoneal injection, blood gas analysis and blood glucose were detected of 4 rats in each group. The other 8 rats in each group were subjected to Morris water maze to access spatial learning and memory function at age of 28 d and decapitated immediately after the tests. The CA1 region of hippocampus were isolated and sliced for recording of field excitatory postsynaptic potential （fEPSP） and the success rate of LTP. RESULTS The indexes of blood gas analysis and blood glucose of 3 groups were in normal range. Compared with that of the group C and P1, escape latency and path length of group P2 were significantly increased from the d 3 of training test （P 〈 0.05）, while the time searching platform in target quadrant and the times crossing over the former platform location were decreased （P〈 0.05）. There were no significant differences between group C and P1 （P 〉 0.05）. After the conditioning stimulation, the slope of fEPSP in the group P1 and P2 were lower than that in the group C （P 〈 0.05）. Furthermore, compared with that of group P1, the slope of fEPSP were reduced in the group P2 （P 〈 0.05）. The success rates of LTP were significantly reduced in the group P2 （P 〈 0.05）, and showed no differences between group C and P1 （P 〉 0.05）. CONCLUSION Rats exposed to multiple doses of propofol inhibites the formation of LTP, which lead to deficit in learning an

关 键 词：丙泊酚 海马 学习 记忆 长时程增强 

分 类 号：R971[医药卫生—药品]