东北刺人参愈伤组织诱导和继代的研究  

Studies on the Callus Inducement and Subculture of Oplopanax elaus Nakai

作　　者：葛江丽[1] 李成浩[2] 刘芳[1] 施汉玉 栾泰龙[1] 刘瑰琦[1] 郑焕春[1] 崔巍[1] 

机构地区：[1]黑龙江省牡丹江林业科学研究所,黑龙江牡丹江157009 [2]东北林业大学,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2012年第30期14660-14660,14667,共2页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省森林工业总局青年基金项目(sgzjQ2010001)

摘　　要：[目的]研究东北刺人参愈伤组织的诱导和继代方法。[方法]以取自长白山的刺人参植株为材料,研究不同外植体、培养基、激素对刺人参愈伤组织诱导的影响。[结果]叶片为刺人参组织培养的最适宜外植体;5月为外植体的最佳取材时期;以MS为基本培养基,并添加1.0 mg/L 2,4-D和1.0 mg/L 6-BA的培养基中愈伤组织诱导率最高;最佳继代培养基为MS+6-BA 2.0 mg/L+NAA 0.2mg/L。[结论]东北刺人参的最适愈伤诱导培养基和继代培养基分别是:MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L和MS+6-BA 2.0 mg/L+NAA 0.2 mg/L。[Objective] To study the callus induction and subculture method of Oplopanax elaus.[Method] With O.elaus as material,the effect of different explants,mediums and hormones on callus induction was studied.[Result] The results showed that leaf was the best suitable organs for tissue culture.May was the best period to draw materials.MS added to 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA had high induction rate on callus;and the optimal subculture medium was MS＋6-BA 2.0 mg/L＋ NAA 0.2 mg/L.[Conclusion] The best callus induction medium and ubculture medium of O.elaus： MS＋2,4-D 1.0 mg/L＋6-BA 1.0 mg/L and MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L.

关 键 词：东北刺人参 组织培养 愈伤组织 诱导条件 继代 

分 类 号：S567.51[农业科学—中草药栽培]