地衣芽孢杆菌BL03脂肪酶基因LIP的克隆及序列分析  

Cloning and Sequence Analysis of LIP Gene from Bacillus Licheniformis BL03

作　　者：刘生峰[1,2] 肖进文[1] 周蓓莉[1,3] 周庆[1] 聂福平[1] 王昱[1] 杨俊[1] 李应国[1,2] 

机构地区：[1]重庆出入境检验检疫局,重庆400020 [2]重庆市进出口食品安全工程研究中心,重庆400020 [3]西南大学,重庆400715

出　　处：《安徽农业科学》2012年第30期14661-14662,14681,共3页Journal of Anhui Agricultural Sciences

基　　金：国家质检总局公益性项目(201010045);重庆市科技攻关项目(CSTC)(2011AC1056;2011AC1057)

摘　　要：[目的]克隆地衣芽孢杆菌BL03的脂肪酶基因LIP并对其序列进行分析。[方法]以地衣芽孢杆菌BL03基因组核酸为模板,PCR扩增得到地衣芽孢杆菌脂肪酶基因,用pMD19-T载体克隆后进行PCR鉴定及序列测定和生物信息学分析。[结果]测序结果表明该片段完整开放阅读框大小为615 bp,与已发表的地衣芽孢杆菌LTP基因(GeneID:3098655)相比,核酸序列相似为99.67%;推导编码氨基酸序列相似性为99.02%。[结论]该基因的获得为其异源表达及相关研究奠定了基础。[Objective] To clone Bacillus licheniformis BL03 gene LIP,and analyze the sequence.[Method] The LIP gene encoding lipase from B.licheniformis BL03 was amplified by PCR and cloned to the vector pMD19-T.[Result] Nucleotide sequence analysis of the LIP gene revealed that the coding region was formed of one complete ORF including 615 base pairs.The cloned gene was 99.67% similar with B.licheniformis LIP gene（GeneID：3098655） and the putative amino acid sequence was 99.02% similar respectively.[Conclusion] The cloned gene laid a foundation for the heterologous expression and relevant researches.

关 键 词：地衣芽孢杆菌 脂肪酶基因 克隆 序列分析 

分 类 号：S182[农业科学—农业基础科学] Q933[生物学—微生物学]