蛇足石杉体内抑制乙酰胆碱酯酶活性物质的内生真菌的分离  

Isolation of Endophytic Fungi with Acetylcholinesterase Inhibitory Activities from Huperzia serrata

作　　者：凌庆枝[1] 董丽辉[1] 范三微[1] 何军邀[1] 高莉莉[1] 黄贝贝[1] 魏兆军[2] 

机构地区：[1]浙江医药高等专科学校,浙江宁波315100 [2]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《安徽农业科学》2012年第30期14706-14707,14712,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省科技厅科研计划项目(2009C33154);宁波市生物医药人才基地项目(Jd080202)资助课题

摘　　要：[目的]从蛇足石杉体内分离筛选出具有体外抑制乙酰胆碱酯酶活性的内生真菌。[方法]取野外采集的蛇足石杉的叶、茎和根进行表面消毒后接种于PDA培养基,分离出内生真菌后进行摇瓶发酵,超声提取其发酵产物,以DTNB法检测其发酵产物在体外抑制乙酰胆碱酯酶的活性。[结果]从根、茎、叶中分离得到194株内生真菌,在马铃薯葡萄糖液体培养基(PDB)中发酵后,对内生真菌发酵提取液进行体外AChE抑制活性检测;共有31株内生真菌显示出体外AChE抑制活性,抑制活性较强的有13株,抑制率在50.2%～80.5%。其中从蛇足石杉茎部位分离得到9株,叶部位分离得到4株。[结论]从蛇足石杉体内可分离出内生真菌,该内生真菌的发酵产物在体外有较强的抑制乙酰胆碱酯酶活性,这可能是寻找乙酰胆碱酯酶抑制药物的有效途径之一。[Objective] To isolate endophytic fungi with acetylcholinesterase（AChE） inhibitory activities from Huperzia serrata（Thunb.）Trev.[Method] Stems,leaves and roots of H.serrata were collected from the open field.Then,they were inoculated on PDA culture medium after the surface sterilization.Based on the isolation of endophytic fungi,shaking flask fermentation was conducted;and fermentation products were extracted by ultrasound.Inhibitory effects in vitro of fermentation products on AChE were detected by DTNB method.[Result] A total of 194 endophytic fungi were isolated from the roots,stems and leaves.After the fermentation in potato glucose liquid medium（PDB）,in vitro AChE inhibitory activity was detected in endophytic fungi fermentation extracting liquid.31 endophytic fungi showed in vitro AChE inhibitory activity.Among them,13 strains had relatively strong inhibitory activity,with the inhibitory rate being 50.2%-80.5%.9 strains were isolated from the H.serrata stems;and 4 strains were isolated from leaves.[Conclusion] Endophytic fungi could be isolated from the H.serrata.The fermentation products of the endophytic fungi showed relatively strong in vitro inhibitory activity to AChE,which might be one of the effective approaches to find the inhibitory drugs of AChE.

关 键 词：蛇足石杉[Huperzia serrata(Thunb.)Trev.] 内生真菌 乙酰胆碱酯酶 抑制 

分 类 号：S567[农业科学—中草药栽培]