敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达研究  

Study on the Differential Expression of Immune Genes between Body Side Skin and Groin Skin of Aohan Fine Wool Sheep

作　　者：赵金山[1,2] 李和刚[2] 刘开东[2] 柳楠[3] 李金泉[1] 

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010018 [2]山东省青岛市畜牧兽医研究所,山东青岛266100 [3]青岛农业大学,山东青岛266019

出　　处：《安徽农业科学》2012年第30期14731-14733,14740,共4页Journal of Anhui Agricultural Sciences

基　　金：国家绒毛用羊产业技术体系资助项目(CARS-40)

摘　　要：[目的]从免疫基因中挖掘调控羊毛性状的候选主效基因。[方法]利用芯片技术,检测敖汉细毛羊体侧部与腹股沟部皮肤免疫基因的差异表达。[结果]差异倍数在2.0以上的免疫基因共46个,并对其中6个基因进行了实时定量PCR验证,其结果表明芯片数据和实时定量PCR验证结果相符率达66.67%,说明芯片数据是可靠的。[结论]免疫赦免机制可能参与羊毛的生长调控。[Objective] The research aimed to find out candidate major genes for regulating the wool traits from immune genes.[Method] Using microarray technology,the differential expression of immune genes between body side skin and groin skin of Aohan fine wool sheep was detected.[Result] 46 immune genes（difference multiple）2.0） were identified and classified.And 6 of them were validated by real-time quantitative PCR.The results showed that the consistency between microarray data and real-time quantitative PCR results was 66.67%,which indicated that the microarray data was reliable.[Conclusion] The mechanism might be involved in the growth regulation of wool.

关 键 词：敖汉细毛羊 毛囊 免疫基因 差异表达 

分 类 号：S826.81[农业科学—畜牧学]