褪黑激素对慢性应激性抑郁症大鼠海马细胞的影响  

Effects of melatonin on hippocampus cells of rats with chronic mild stress depression

作　　者：薛延军[1] 吴滢[2] 赵涛[2] 周志强[1] 刘益仁[1] 狄荣科[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,212013 [2]江苏大学临床医学院

出　　处：《江苏医药》2012年第20期2378-2380,共3页Jiangsu Medical Journal

摘　　要：目的探讨褪黑激素(MT)对慢性应激性抑郁症大鼠海马细胞的作用及可能机制。方法 36只SD大鼠随机均分为生理盐水(NS)组、抑郁(DM)组和治疗(MT)组。采用Morris水迷宫实验观察MT对大鼠智力行为的影响,用免疫组织化学法测定海马区细胞神经生长因子(NGF)、肿瘤坏死因子α(TNF-α)、MT受体1(MTR-1)、MTR-2及白细胞介素6(IL-6)的表达。结果 MT组大鼠逃避潜伏期和穿越站台次数较DM组减少(P<0.05或P<0.01)。与NS组和DM组相比,MT组TNF-α和IL-6表达减少,而NGF、MTR-1、MTR-2表达增加(P<0.05或P<0.01)。结论MT可能通过增强神经营养素的作用,抑制炎性因子,从而起到抑制抑郁症发作的作用。Objective To investigate the effect and underlying mechanism of melatonin（MT） on the hippocampus cells of rats with chronic mild stress depression（CMSD）. Methods Thirty-six SD rats were equally randomized into three groups of NS（intraperitoneal injection of normal saline）, DM （CMSD model） and MT（CMSD model plus intraperitoneal injection of MT 5.0 mg· kg^-1 · d^-1 ）. The Morris labyrinth test was used for observing the effect of MT on intellectual ability of rats. The expressions of NGF, TNF-α, IL-6, MTR-1 and MTR-2 in the hippocampus of rats were detected by immunohistochemistry. Results The escape latency and fault frequency in group MT were less than those in group DM（P〈0. 05 or P〈0. 01）. Compared with groups of NS and DM, the expressions of TNF-α and IL-6 were decreased, while the expressions of NGF, MTR-1 and MTR-2 were increased in group MT （P〈0. 05 or P〈0. 01 ）. Conclusion MT palys a role in anti-depression probably by enhancing the nervous protection of nutrients and inhibiting the inflammatory factors.

关 键 词：慢性轻度应激性抑郁 褪黑激素 海马 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]