鸡前脂肪细胞中A-FABP基因表达的变化对PPARγ、perilipin和E-FABP表达的影响  

The Effect of A-FABP Gene Overexpression or Knockdown on the Expression of PPARγ,perilipin and E-FABP in Chicken Adipocytes

作　　者：张庆秋[1,2] 石慧[1,2] 王宇祥[1,2] 李辉[1,2] 王启贵[1,2] 

机构地区：[1]农业部鸡遗传育种重点实验室,哈尔滨150030 [2]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《畜牧兽医学报》2012年第10期1531-1538,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30771542;30972087);现代农业产业技术体系建设项目(CARS-42);黑龙江省高等学校科技创新团队建设项目(2010td02)

摘　　要：本研究以鸡原代前脂肪细胞为实验材料,探讨A-FABP基因在鸡脂类代谢过程中的功能以及该基因与脂类代谢相关7个基因间的调控关系。利用A-FABP基因的shRNA干扰载体和真核表达载体对该基因进行干扰和过表达,分别在干扰和过表达后24、36、48、60和72h时检测脂类代谢相关基因的表达情况。结果表明,干扰A-FABP后,鸡前脂肪细胞中PPARγ和perilipin基因的表达量在24和36h时显著低于对照组,在48h时显著高于对照组,在48和72h时,E-FABP基因表达量显著低于对照组;过表达A-FABP后,鸡前脂肪细胞中PPARγ和perilipin基因的表达量在24和36h时显著高于对照组,在48h时显著低于对照组,在所有检测的时间点E-FABP基因表达均显著下调。结果显示,在前脂肪细胞中,鸡A-FABP表达量的变化影响PPARγ、perilipin和E-FABP的表达。This study was designed to investigate the role of chicken A-FABP gene in lipid metab- olism of adipocytes and the regulation relationship between A-FABP gene and the genes related to lipid metabolism. The shRNA interference vector with A-FABP gene and eukaryotic expres- sion vector were used. Chicken adipocytes with short hairpin RNA-mediated knockdown and A-FABP cDNA overexpression were used. The genes expression level in chicken adipocytes were detected at 24, 36, 48, 60 and 72 h after transfection with the interference and overexpression plasmids of A-FABP. The results showed that the expression levels of PPAR？, and perilipin genes were downregulated at 24 and 36 h and upregulated at 48 h in the A-FABP interference group. The expression levels of E-FABP genes was downregulated at 48 and 72 h in the A-FABP interference group. The expression levels of PPARy and perilipin genes were upregulated at 24 and 36 h and downregulated at 48 h in the A-FABP overexpression group. The expression levels of E-FABP genes was downregulated at all detected time pionts in the A-FABP overexpression group. These results indicated that A-FABP might affect expression of PPARγ, perilipin and E-FABP in chicken adipocytes.

关 键 词：A-FABP基因 PPARΓ基因 perilipin基因 E-FABP基因 鸡 脂肪细胞 

分 类 号：S831.2[农业科学—畜牧学]