绒山羊骨骼肌miRNAs及其靶基因表达谱分析  

The Analysis of miRNAs and Their Target Expression Profiles in Cashmere Goat Skeletal Muscle

作　　者：韩志玲[1] 赵德超[1] 付绍印[1] 吕晓曼[1] 高爱琴[1] 张文广[1] 李金泉[1] 

机构地区：[1]内蒙古农业大学动物科学学院动物遗传育种与繁殖自治区重点实验室,呼和浩特010018

出　　处：《畜牧兽医学报》2012年第10期1539-1546,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑项目(2011BAD28B05);国家自然科学基金(30960246);现代农业产业技术体系建设专项资金(CARS-40-05);公益性行业(农业)科研项目(201003019)

摘　　要：本试验选取了miR-1、miR-133、miR-24、miR-122、miR-103、miR-143、let7 7个miRNAs,旨在对其表达量及靶基因表达谱进行分析。选用4只绒山羊的背部和后腿肌肉提取RNA,反转录后,利用SYBR荧光定量PCR来检测各miRNAs的表达量,并采用靶基因指纹图谱(MTFP)技术获得各miRNAs的靶基因表达谱。结果表明,miR-NA表达量依次为:miR-1>miR-133>miR-24>let7>miR-143>miR-103>miR-122,其中miR-1和miR-133表达规律相似,且这7个miRNAs在成羊中的表达量均高于羔羊;此外,miRNAs表达量的差异还受性别影响,且miR-NA表达量与靶基因个数及其表达量之间具有相关性。对5个miRNAs的10个靶基因测序并比对,表明靶基因分别编码与信号转导、细胞周期等相关的蛋白。通过这7个miRNAs与其靶基因表达的分析,揭示了肌肉发育相关miRNAs及脂肪发育相关miRNAs在绒山羊骨骼肌中的异同,有利于绒山羊肉质基因的研究。Seven muscle and adipose related miRNAs, including miR-1, miR-133, miR-24, miR- 122, miR-103, miR-143 and let7 were selected to analyze their expression and target expression profiles. Muscles RNA from back and hind leg muscle of 4 cashmere goats were extracted, after reverse-transcription, SYBR TaqMan PCR was used to detect gene expression, and miRNA tar- get expression profiles were got by PAGE electrophoresis and MiRNA Targets Figure Print （MTFP） technique. The result showed that the expression of miRNA were as follows：miR-l〉 miR-133〉miR-24〉let7〉miR-143〉miR-103〉miR-122, the expression patterns of miR-1 and miR-133 were similar,and the expression levels of all miRNAs in adult goat were higher than that in lamb; moreover, the difference of miRNAs expression levels were also affected by gender, and they had relationship with number and expression levels of target genes. 10 target genes in 5 miRNAs were sequenced and Blast annotation, which coded proteins related to signal transduc- tion, ceil cycle, and so on. The expression analysis of 7 miRNAs and their target genes showed differences and similarities between muscle development related miRNAs and adipose develop- ment related miRNAs in cashmere goat skeletal muscle, which were favorahle to the research for meat quality related genes in cashmere goat.

关 键 词：MIRNA 靶基因 表达 绒山羊 骨骼肌 

分 类 号：S827[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]