与鸡传染性支气管炎病毒混合感染状况下鸭源鸡杆菌在鸡体内的分布  

Tissue Distribution of Gallibacterium anatis in Chickens Co-infected with Infectious Bronchitis Virus

作　　者：皇甫和平[1,2] 赵军[1] 杨霞[1] 李乔晶[1] 王川庆[1] 陈陆[1] 常洪涛[1] 王新卫[1] 刘红英[1] 姚慧霞[1] 

机构地区：[1]河南农业大学禽病研究所,郑州450002 [2]郑州牧业工程高等专科学校,郑州450011

出　　处：《畜牧兽医学报》2012年第10期1623-1629,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：柏林格公司项目(43006167);国家自然科学基金(30972187)

摘　　要：为研究鸭源鸡杆菌(Gallibacterium anatis)在鸡体内的动态分布及鸡传染性支气管炎病毒(Infectious bronchitis virus,IBV)对其的影响,将鸭源鸡杆菌和鸡传染性支气管炎病毒分别或同时人工接种SPF蛋鸡,分别于接种后12、24、48、72、96h对鸡进行剖检,无菌采集鸡的10种组织样品(上腭裂、气管、肺脏、心脏、脾脏、卵巢、肾脏、肝脏、十二指肠和输卵管),利用SYBR Green I定量PCR(Quantitative PCR,qPCR)方法检测鸭源鸡杆菌组、混合感染组在不同时间点、不同器官中的鸭源鸡杆菌DNA含量。结果显示:鸭源鸡杆菌单独感染时,12h时即可侵袭到气管、心脏、脾脏、卵巢和肾脏,24h时便可感染肝脏、十二指肠和输卵管,48h时可传播到肺脏,72h时感染上腭裂。混合感染组结果表明,鸭源鸡杆菌12h到达卵巢,24h时即可出现在所有器官。混合感染组各器官的总体qPCR检出率为37.1%,显著高于鸭源鸡杆菌组的25.3%。鸭源鸡杆菌组和混合感染组qPCR检测结果均显示,鸭源鸡杆菌DNA在气管中的检出率最高,在卵巢中达到最高含量。鸭源鸡杆菌能够造成试验鸡的全身感染,该菌主要对鸡的呼吸系统和生殖系统有致病性,气管和卵巢是其主要的致病器官;2种病原同时接种加重了全身感染,IBV促进了鸭源鸡杆菌在鸡体内的传播,尤其促进了其在十二指肠中的增殖,增强了鸭源鸡杆菌对消化系统的致病性,导致鸡腹泻的发生。The objective of this study was to understand the dynamic distribution pattern of G. anatis in chicken and the effect of chicken infectious bronchitis virus （IBV） on the distribution pattern. In the present study, SPF layer chickens were inoculated with G. anatis or/and chicken infectious bronchitis virus （IBV）. Quantitative PCR （qPCR） with SYBR Green I was used to detect the DNA of G. anatis in different organs at different time point from chicken after artificial infection. In G. anatis group, G. anatis DNA was firstly detected in the trachea, heart, spleen, ovary and kidney of chickens 12 hours post inoculation （PI）, in the liver, duodenum and oviduct 24 hours PI, in the lung 48 hours PI, and in the palate cleft 72 hours PI, respectively. In-mixed infection group, G. anatis DNA was detected 12 hours PI in the ovaries, and 24 hours PI in all the organs. The highest content of G. anatis DNA was noticed in ovary and the highest detection rate was found in trachea of birds. Mixed-infection group had significantly higher qPCR detection rate of all organs than G. anatis group. G. anatis could cause systemic infection of the birds, and coinoculation of G. anatis and IBV increased the systemic infection. IBV promoted the spread of G. anatis, moreover, IBV benefited the multiplication and pathogenicity of G. anatis in the duo- denum and thus resulted in clinical diarrhea. It was further confirmed that the pathogenicity of G. anatis mainly focus on the respiratory and reproductive systems. Trachea and ovaries were the target organs of G. anatis.

关 键 词：鸭源鸡杆菌 SPF蛋鸡 动态分布 传染性支气管炎病毒 定量PCR 人工感染 

分 类 号：S852.659.6[农业科学—基础兽医学] S852.612[农业科学—兽医学]