猪链球菌-副猪嗜血杆菌二联亚单位疫苗在小鼠的免疫效力分析  

Analysis of Immune Effect on Streptococcus suis-Haemophilus parasuis Bivalent Subunit Vaccine in Mice

作　　者：赵建平[1] 魏燕鸣[1] 宋岱松[1] 张强[1] 朱剑[1] 周明光[1] 李冉[1] 康超[1] 金梅林[1] 

机构地区：[1]华中农业大学农业微生物国家重点实验室,武汉430070

出　　处：《畜牧兽医学报》2012年第10期1639-1644,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家863计划(2011AA10A210);国家973计划(2012CB518805)

摘　　要：本研究旨在开发一种用于防制猪链球菌病和副猪嗜血杆菌病的新型二联基因工程疫苗,并对其免疫效应进行评价。选择猪链球菌2型蛋白溶血素SLY、HP0197和副猪嗜血杆菌外膜蛋白D15和HPS06257 4种蛋白,重组表达并纯化,添加一定比例的佐剂制成含有不同组分的亚单位疫苗。研究表明该二联亚单位疫苗及各单苗能够引起小鼠良好的体液免疫,并能保护机体分别抵抗致死剂量猪链球菌2型(SS2)和副猪嗜血杆菌(HPS)强毒株的攻击。此外,调理吞噬试验表明疫苗高免血清能够有效杀死细菌,并赋予机体被动抵抗SS2和HPS感染的能力。研究证实制备的猪链球菌-副猪嗜血杆菌二联亚单位疫苗在小鼠具备良好的免疫保护效力。This study aims to develop a new kind of bivalent genetic engineering vaccine used to control Streptococcus suis （SS） disease and Haernophilus parasuis （HPS） disease, and to evalu- ate the vaccine＇s immune effect. Four proteins, Streptococcus suis serotype 2 proteins SLY, HP0197, and Haernophilus parasuis outer membrane proteins D15 and HPS06257 are selected, recombinant expressed, purified and added adjuvant of certain proportion to be made with differ- ent components of subunit vaccines. In the present study, the Streptococcus suis serotype 2-Hae- mophilus parasuis （SS2-HPS） bivalent subunit vaccine and their single vaccines could elicit a sig- nificant humoral antibody response and confer significant protection against challenge with lethal dose of SS2 or HPS in mice, respectively. In addition, the hyperimmune sera against these vac- cines could efficiently kill the bacteria in the opsonized phagocytosis test and confer significant protection against SS2 infection in the experiment of passive immunization. The study shows this SS-HPS bivalent subunit vaccine has good immune protection in mice.

关 键 词：猪链球菌2型 副猪嗜血杆菌 二联亚单位疫苗 调理吞噬试验 免疫保护 

分 类 号：S852.61[农业科学—基础兽医学]