大熊猫等8种野生哺乳动物蛔虫的线粒体COXⅠ和COXⅡ基因的亲缘关系分析  

Genetic Relationship of Ascarid Nematodes from Giant Panda and Seven Other Species of Captive Wild Mammals Based on COXⅠ and COXⅡ Genes

作　　者：牛李丽[1] 陈世界[2] 汪涛[3] 古小彬[3] 严玉宝[2] 余华[2] 邓家波[1] 严慧娟[1] 余星明[1] 陈维刚[1] 王淑贤[3] 杨光友[3] 

机构地区：[1]成都动物园,成都610041 [2]四川出入境检验检疫局,成都610041 [3]四川农业大学动物医学院,雅安625014

出　　处：《畜牧兽医学报》2012年第10期1645-1650,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：成都大熊猫繁育研究基金项目(CPF-08005);科技部公益项目(200910188);国家质检总局项目(2010IK004)

摘　　要：为了探讨寄生于大熊猫、小熊猫、北极熊、棕熊东北亚种、棕熊西藏亚种、黑熊四川亚种、黑猩猩和白眉长臂猿体内蛔虫的分类地位,采用PCR技术扩增了这些野生动物体内寄生蛔虫的线粒体COXⅠ、COXⅡ基因序列,并与GenBank中注册的同源性序列进行了分析。序列分析结果显示:扩增的8种野生哺乳动物蛔虫的COXⅠ、COXⅡ基因长度均分别为393和582bp,其中大熊猫与小熊猫及4种熊科动物蛔虫COXⅠ和COXⅡ基因的相似性分别为94.8%～95.0%和94.9%～95.5%;黑猩猩和白眉长臂猿蛔虫的COXⅠ、COXⅡ基因的相似性分别为99.8%和99.5%。分子系统树(NJ/MP/ML)表明,寄生在大熊猫、小熊猫和4种熊科动物体内的蛔虫均为贝蛔属(Baylisascaris)蛔虫;而黑猩猩和白眉长臂猿体内寄生的蛔虫应为蛔属(Ascaris)蛔虫。同时,分析结果也揭示了蛔虫与宿主之间存在协同进化关系。The aim of this study was to investigate the taxonomic relationships of ascarid nema- todes from Ailuropoda rnelanoleuca, Ailurus fulgens, Ursus rnaritimus, Ursus arctos pruino- sus , Ursus thibetanus mupinensis , Ursus arctos lasiotus , Pan troglodytes and Hylobates hoolock. The mitochondrial cytochrome coxidase subunits Ⅰ （COX Ⅰ ） and Ⅱ （COX Ⅱ ） were amplified by PCR and sequenced directly. Then, sequence analyses was carried out based on COX I and COX Ⅱ sequences from ascarid nematodes acquired in our study and 12 homologous sequences from GenBank. The lengths of COX Ⅰ and COX Ⅱ in ascarid nematodes from the eight species of cap- tive animals were all 393 bp and 582 bp, respectively. The COX Ⅰ and COX Ⅱ of ascarid nema- todes from giant panda shared 94. 8%-95.0% and 94.9%-95. 5% sequence identity with these from A. fulgens, U. rnaritimus, U. arctos pruinosus, U. thibetanus mupinensis and U. arctos lasiotus, respectively. Additionally, the COX Ⅰ and COX Ⅱ genes of ascarid nematodes from P. troglodytes shared 99.8 % and 99.5 % identity with the nematodes from H. hooloc, respectively. Phylogentic analysis showed that ascarid nematodes from A. rnelanoleuca, A. fulgens and four Ursids species belonged to the genus Baylisascaris, while roundworms from H. hoolock and P. troglodytes clustered under the genus Ascaris. These results showed that a co-evolutionary rela- tionship may link the ascarid nematodes and their corresponding hosts together.

关 键 词：大熊猫 野生哺乳动物 蛔虫 线粒体 COXⅠ COXⅡ 亲缘关系 

分 类 号：S852.73[农业科学—基础兽医学]