小型猪特异性麻醉颉颃剂对XFM麻醉下大鼠海马c-jun基因mRNA转录的影响  

Effects of the Specificity Antagonist for Miniature Pigs Combined Anaesthetic on the Transcription of c-jun Gene mRNA in the Hippocampus of Rats Anesthetized with XFM

作　　者：李小波[1] 尹柏双[1,2] 李志强[1] 石星星[1] 范宏刚[1] 王洪斌[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]吉林农业科技学院动物医学学院,吉林132101

出　　处：《畜牧兽医学报》2012年第10期1664-1668,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30671552;30972227)

摘　　要：本试验旨在研究小型猪特异性麻醉颉颃剂对小型猪特异性麻醉剂(XFM)麻醉下大鼠海马脑区c-jun基因mRNA转录的影响,藉以探究其催醒机制。90只成年SD大鼠被随机分成XFM麻醉组、XFM麻醉+生理盐水组、XFM麻醉+小型猪特异性麻醉颉颃剂组,每组分为5个不同采样时间点的亚组。采用实时荧光定量PCR技术检测样品中c-jun基因mRNA转录量。结果显示,XFM麻醉诱导大鼠海马脑区c-jun基因mRNA转录,各时间点海马脑区c-jun基因mRNA转录与0min比较显著升高(P<0.01),XFM麻醉大鼠腹腔注射生理盐水后各时间点海马脑区c-jun基因mRNA转录与XFM对照组间无显著差异(P>0.05),注射小型猪特异性麻醉颉颃剂后抑制了XFM诱导大鼠海马c-jun基因mRNA的转录,与XFM对照组比较差异显著(P<0.01或P<0.05)。结果表明,小型猪特异性麻醉颉颃剂抑制了XFM麻醉诱导大鼠海马脑区c-jun基因mRNA的转录,这可能与小型猪特异性麻醉颉颃剂催醒机制相关。To explore the antagonism mechanism of the specificity antagonist for miniature pigs combined anaesthetic, the effect of the specificity antagonist on the c-jun gene transcription in the hippocampus of rats anesthetized with XFM was studied in this experiment. Ninety adult SD rats were divided randomly into three groups： XFM group, XFM add saline group and XFM add the specificity antagonist group. Each group consists of five subgroups, and samples were collected at different time points. The transcription of c-jun gene mRNA in every sample was detected with real time PCR. The result showed that The c-jun gene mRNA contents in each time point of the XFM group were obviously increased as compared with 0 rain （P〈0.01）. There were no signifi- cant effect of injection of saline after XFM administrated on the c-jun gene mRNA transcription （P〈0.05）. The level of c-jun gene mRNA transcription after the specificity antagonist adminis- trated was significantly lower than XFM group （P〈0.01 or P〈0.05）. These results indicated that the specificity antagonist for miniature pigs combined anaesthetic attenuates the c-jun gene mRNA transcription in the hippocampus of rats induced by XFM, this may be related to the an- tagonism mechanism of the specificity antagonist for miniature pigs＇ combined Anaesthetic.

关 键 词：小型猪特异性麻醉颉颃剂 海马 c-jun 催醒机制 

分 类 号：S852.979.1[农业科学—基础兽医学]