氮源和碳源对解淀粉芽孢杆菌Q-426抗菌脂肽合成的影响  

Effects of Different Nitrogen and Carbon Sources on the Production of Antifungal Lipopeptides from Bacillus amyloliquefaciens Q-426

作　　者：赵朋超[1,2] 权春善[3,4] 金黎明[3,4] 王丽娜[1,2] 范圣第[3,4] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]中国科学院研究生院,北京100049 [3]大连民族学院生命科学学院,大连116600 [4]国家民委教育部重点实验室,大连116600

出　　处：《中国生物工程杂志》2012年第10期50-56,共7页China Biotechnology

基　　金：辽宁省教育厅重点实验室项目(LS2010049)资助项目

摘　　要：微生物来源的环状脂肽在生物农药以及医学领域极具应用潜力。通过测量抑菌活性并利用反相高效液相色谱(RP-HPLC),液相质谱联用(HPLC-MS)和串联质谱(MS/MS)技术检测抗菌脂肽组分的变化研究了氮(N)源、碳(C)源和培养基中的初始pH值对解淀粉芽孢杆菌Q-426菌株中抗菌脂肽bacillomycin D和fengycins合成的影响,从而为进一步研究它们生物合成的基因调控以及更有目的性提高产量提供理论依据。结果表明:L-组氨酸、L-赖氨酸、甘油、山梨醇以及培养基中的[OH]-均能促进脂肽bacillomycin D的合成,但它们在该实验菌株抗菌脂肽bacillomycinD合成途径中的调控靶点有所不同。Cyclic lipopeptides,which are produced by several types of microorganisms,will have great potential in the agricultural and biomedical field with their unusual antibacterial,antifungal and antiviral activities.To provide a theoretical basis for investigating the gene regulation of antifungal lipopeptides and enhancing their yield more effectively in the next step,effects of three factors（nitrogen sources,carbon sources and initial pH in the medium） on the production of bacillomycin D and fengycins from Bacillus amyloliquefaciens Q-426 were analyzed by measurement of antifungal activities against Curvularia lunata（Walk） Boed,reverse-phase high performance liquid chromatography（RP-HPLC）,HPLC-mass spectrometry（MS） and MS/MS.Finally,the results were screened as follows： L-histine,L-lysine,glycerol,sorbitol and-in the medium could promote the accumulation of bacillomycin D,but less effective to that of fengycins.However,further analysis of RP-HPLC spectrum revealed the three factors above played different roles in the pathway of bacillomycin D biosynthesis from strain Q-426.

关 键 词：解淀粉芽孢杆菌Q-426 Bacillomycin D Fengycins 碳源 氮源 

分 类 号：Q819[生物学—生物工程]