Effects of Epidermal Growth Factor and β-mercaptoethanol on In-vitro Fertilization and Development of Oocytes from Hormone-Stimulated Lambs  

作　　者：GUO Hong1,2,3,WAN Peng-cheng2,3SHI Wen-yan4,NI Jian-hong2,3,DAI Rong2,3,MAO Qing-qing2,3,5,ZHU Hui1,2,3,ZHANG Bin2,3,LIU Chang-bin2,3,SHI Guo-qing2,3 1. College of Life Sciences,Shihezi University,Shihezi 832000,China 2. Animal Husbandry and Veterinary Institute,Xinjiang Academy of Agri- cultural and Reclamation Science,Shihezi 832000,China 3. Key Lab of Sheep Breeding and Reproduction,Xinjiang Agroreclamation Academy of Sciences,Shihezi 832000,China 4. Medical College,Shihezi University,Shihezi 832000,China 5. College of Animal Science & Technolo- gy,Nanjing Agricultural University,Nanjing 210095,China 

出　　处：《Animal Husbandry and Feed Science》2012年第2期49-52,共4页动物与饲料科学（英文版）

基　　金：funded by the National Natural Science Foundation of China (31160460);Doctor Fund of Xinjiang Military Groups (2010JC10);National Wool Industry System (nycytx-40-06);National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2011BAD28B05-1-1);863 Plan (2011AA100307);Breeding Strategy of Xinjiang Group (2011BA006)

摘　　要：[Objective]The aim was to explore optimization of system of oocytes in-vitro culture of young animals. [Method]Effects of EGF （ epidermal growth factor） and β-mercaptoethanol in maturation media on fertilization,cleavage and blastaea were researched,which were then compared with those of adult sheep. [Result]EGF in different concentrations had little effects on rates of cleavage and blastaea （ P 〉0.05） and β-mer-captoethanol in different concentrations would improve blastaea rate of oocytes,for example,100 μmol/L of β-mercaptoethanol has significant effects on balstaea rate （ P 〈0.05） ,but has little effects on cleavage rate （ P 〉0. 05） . In addition,rates of cleavage and balstaea of oocytes in lamb were both lower than those of adult sheep （ P 〈0.05） ; fertilization rate of oocytes in lamb （ P 〈0.05） ,which differed little with that of adult sheep （ P 〉0.05） ,could be significantly enhanced by 100 μmol/L of β-mercaptoethanol. Furthermore,polyspermy rate was higher than that of adult sheep without β-mercaptoethanol （ P 〈0.05） ; the rate was of little differences with that of adult sheep with 100 μmol/L of β-mercaptoethanol （ P 〉 0.05） ; unfertilization rate （ 20%） in media without β-mercaptoethanol was a little higher （ P 〉0.05） than those of adult sheep （ 12.3%） and those in media with β-mercaptoethanol （ 13.5%） . [Conclusion]Developmental capacity of oocytes and fertilization rate could be improved by 100 μmol/L of β-mercaptoethanol with polyspermy rate reduced,but developmental capacity of lamb was significantly lower than that of adult sheep.[Objective]The aim was to explore optimization of system of oocytes in-vitro culture of young animals. [Method]Effects of EGF （ epidermal growth factor） and β-mercaptoethanol in maturation media on fertilization,cleavage and blastaea were researched,which were then compared with those of adult sheep. [Result]EGF in different concentrations had little effects on rates of cleavage and blastaea （ P 〉0.05） and β-mer-captoethanol in different concentrations would improve blastaea rate of oocytes,for example,100 μmol/L of β-mercaptoethanol has significant effects on balstaea rate （ P 〈0.05） ,but has little effects on cleavage rate （ P 〉0. 05） . In addition,rates of cleavage and balstaea of oocytes in lamb were both lower than those of adult sheep （ P 〈0.05） ; fertilization rate of oocytes in lamb （ P 〈0.05） ,which differed little with that of adult sheep （ P 〉0.05） ,could be significantly enhanced by 100 μmol/L of β-mercaptoethanol. Furthermore,polyspermy rate was higher than that of adult sheep without β-mercaptoethanol （ P 〈0.05） ; the rate was of little differences with that of adult sheep with 100 μmol/L of β-mercaptoethanol （ P 〉 0.05） ; unfertilization rate （ 20%） in media without β-mercaptoethanol was a little higher （ P 〉0.05） than those of adult sheep （ 12.3%） and those in media with β-mercaptoethanol （ 13.5%） . [Conclusion]Developmental capacity of oocytes and fertilization rate could be improved by 100 μmol/L of β-mercaptoethanol with polyspermy rate reduced,but developmental capacity of lamb was significantly lower than that of adult sheep.

关 键 词：Lamb Oocyte In-vitro fertilization EGF β-mercaptoethanol 

分 类 号：S826[农业科学—畜牧学]