结核分枝杆菌氟喹诺酮耐药与gyrA基因突变的关系  

Characterization of gyrA gene mutation in fluoroquinolones-resistant Mycobacterium tuberculosis

作　　者：杨辉[1,2] 张国良[1] 张明霞[1] 吴爱武[3] 陈建波[4] 陈心春[1] 

机构地区：[1]广东省深圳市第三人民医院肝研所,518112 [2]广州医学院 [3]广州医学院第一附属医院检验科,510120 [4]广东省深圳市第三人民医院检验科,518112

出　　处：《实用医学杂志》2012年第20期3457-3459,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81172732)

摘　　要：目的:了解结核分枝杆菌临床分离株氟喹诺酮类药物耐药与gyrA基因突变的关系。方法:选取经过全自动快速分枝杆菌培养鉴定药敏系统(BACTEC-MGIT960)检测的氟喹诺酮类药物敏感株30株,耐药株30株,针对其gyrA基因的氟喹诺酮类药物耐药决定区(QRDR)进行扩增,并将扩增产物进行T-A克隆后进行测序。结果:在这60株结核分枝杆菌临床分离株中,30株敏感株均未在QRDR检出有义突变,30株耐药株中有19株QRDR存在有义突变,主要突变位点为90、91和94。gyrA基因突变占结核分枝杆菌氟喹诺酮类药物耐药菌株的63.3%(19/30)。结论:gyrA基因突变与结核分枝杆菌氟喹诺酮类药物耐药密切相关。Objective To investigate the characterization of gyrA gene mutation in fluoroquinolones-resistant Mycobacterium tuberculosis.Methods 60 Mycobacterium tuberculosis clinical strains,including 30 drug-resistance strains and 30 drug-susceptible strains,were identified and their drug-resistance in phenotype were tested by BACTEC-MGIT960,gyrA gene was amplified by PCR and then fragments were cloned into T-vector and sequenced.Results Among the 30 Mycobacteria tuberculosis drug-susceptible isolates,no sense mutation was identified in QRDR.19 mutant strains at 90,91 or 94 site of gyrA gene were identified in 30 drug-susceptible strains.There were 63.3% strains with gyrA mutation among 30 drug-resistant isolates in phenotype.Conclusion There is closely association between gyrA gene mutation and fluoroqunolones-resistant in phenotype for Mycobacterium tuberculosis.

关 键 词：结核分枝杆菌 氟喹诺酮类 耐药 GYRA 基因突变 

分 类 号：R440[医药卫生—诊断学]