2011年新疆维吾尔自治区辽宁病毒分离鉴定  

Isolation and identification of Liaoning virus in Xinjiang Uygur Autonomous Region,China in 2011

作　　者：唐承军[1] 吕志[1] 付士红[1] 张松[2] 梁国栋[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,传染病预防控制国家重点实验室,北京102206 [2]新疆维吾尔自治区疾病预防控制中心

出　　处：《中国媒介生物学及控制杂志》2012年第5期381-383,387,共4页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(81171635)~~

摘　　要：目的了解新疆维吾尔自治区(新疆)蚊虫携带辽宁病毒情况,为虫媒病毒病防治提供科学依据。方法用镊子夹取法采集蜱虫标本,用诱蚊灯采集蚊虫标本,用细胞培养法分离病毒,并对分离到的病毒进行分子生物学分析。结果用辽宁病毒特异引物对2011年采自喀什地区的蚊虫标本及采自伊犁地区的蜱虫标本进行PCR扩增,结果显示,在蚊虫标本中辽宁病毒核酸阳性率为23.0%(87/379),蜱虫标本中未检测到辽宁病毒核酸;从蚊虫标本C6/36细胞培养物中共分离到19株病毒,经鉴定为辽宁病毒。结论在2011年采自新疆喀什地区的蚊虫标本中分离到多株辽宁病毒;在2011年采自伊犁地区的蜱虫标本中未检测到辽宁病毒;建议在新疆地区开展辽宁病毒对人、畜致病性等方面的研究。Objective To investigate mosquito-borne Liaoning virus （LNV） in Xinjiang Uygur Autonomous Region, China, and to provide evidence for prevention and control of arbovirus diseases. Methods Mosquitoes and ticks were collected from Xinjiang in 2011 and then used for virus isolation by cell culture. The isolated viruses were further analyzed by molecular biology techniques. Results The viruses in the mosquitoes collected from Kashi, Xinjiang and ticks collected from Yili, Xinjiang were subject to PCR using the specific primer for LNV, and the results showed that the positive rate of LNV in mosquitoes was 23.0% （87/379） and that no LNV was found in ticks. Nineteen virus strains were isolated from C6/36 cell cultures of mosquitoes and were identified as LNV. Conclusion Several LNV strains were isolated from mosquitoes collected in Kashi, Xinjiang in 2011, while no LNV was found in ticks collected in Yili, Xinjiang in 2011. Further research on LNV pathogenicity for humans and animals is recommended in Xinjiang, China.

关 键 词：虫媒病毒 辽宁病毒 序列分析 进化分析 

分 类 号：R384.1[医药卫生—医学寄生虫学] R373.3[医药卫生—基础医学]