云南省澜沧江流域蚊虫流行性乙型脑炎病毒分子特征分析  

Analysis of molecular characteristics of Japanese encephalitis virus isolated from mosquitoes in Lancang River watershed in Yunnan province,China

作　　者：郭晓芳[1,2] 王丕玉[2] 曾旭灿[2] 赵秋敏[1] 周传玲[3] 龚道方[3] 李佳[3] 王海波[3] 董利民[2] 周红宁[2] 张久松[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071 [2]云南省寄生虫病防治所,云南普洱665000 [3]大理学院病原与媒介生物研究所(普洱分部)

出　　处：《中国媒介生物学及控制杂志》2012年第5期391-394,共4页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(30660160)~~

摘　　要：目的确定2007-2009年从云南省澜沧江流域采集蚊虫中分离的13株流行性乙型脑炎病毒(JEV)基因型及其E基因序列特征。方法用反转录-聚合酶链反应(RTPCR)扩增E蛋白核苷酸序列,测序后用DNAStar软件包中的MegAlign程序完成核苷酸相似性和氨基酸同源性分析;用ClustalX1.83软件进行碱基配对后采用Mega5.0软件做进化树分析。结果 13株JEVE基因的核苷酸相似性为98.0%～100%,氨基酸的同源性为99.8%～100%,与我国乙脑减毒活疫苗株SA14-14-2的核苷酸相似性为86.1%～86.9%,氨基酸同源性为96.8%～97.2%;进化树分析发现,13株JEV均属于基因Ⅰ型。结论 2007-2009年从云南省澜沧江流域采集蚊虫中分离的13株病毒属于JEV基因Ⅰ型。Objective To analyze the genotypes and E gene sequence characteristics of 13 Japanese encephalitis virus （JEV） strains isolated from mosquitoes in Lancang River watershed in Yunnan province, China during 2007-2009. Methods E genes of 13 JEV strains were amplified by RT-PCR. The nucleotide similarity and amino acid homology were analyzed by MegAlign program in DNAStar software. Multiple sequence alignment and phylogenetic analysis were carried out using Clustal X 1.83 and the Mega 5.0. Results The nucleotide similarity and amino acid homology among the 13 JEV strains were 98.0%-100% and 99.8%-100%, respectively. Compared with the live attenuated vaccine strain （SA-14-14-2）, the 13 JEV strains showed nucleotide similarity and amino acid homology of 86.1%-86.9% and 96.8%-97.2%, respectively. Phylogenetic analysis showed that the 13 JEV strains were genotype I. Conclusion The 13 JEV strains isolated from mosquitoes in Lancang River watershed in Yunnan province, China durin~ 2007-2009 belonged to genotvpe I.

关 键 词：澜沧江流域 流行性乙型脑炎病毒 基因Ⅰ型 

分 类 号：R384.1[医药卫生—医学寄生虫学] R512.32[医药卫生—基础医学]