云南省弥勒县2009年蚊媒病毒分离和鉴定  

Isolation and identification of mosquito-borne viruses in Mile county,Yunnan province,China in 2009

作　　者：冯云[1] 陈卫武 杨卫红[1] 章域震[1] 杨杜鹃[1] 刘芬[1] 张娟[1] 王丕玉[3] 白鹏飞 窦友剑 李继辉 张海林[1] 

机构地区：[1]云南省地方病防治所/云南省病毒立克次体研究中心,云南大理671000 [2]弥勒县疾病预防控制中心,云南弥勒652300 [3]云南省寄生虫病防治所

出　　处：《中国媒介生物学及控制杂志》2012年第5期402-405,共4页Chinese Journal of Vector Biology and Control

基　　金：中美新发和再发传染病合作项目(U19-GH000004);国家自然科学基金(30560142)~~

摘　　要：目的了解云南省红河州弥勒县蚊虫中虫媒病毒的分布特点,为虫媒病毒病防治提供科学依据。方法 2009年7月在弥勒县采集蚊虫标本,用细胞培养法分离病毒,对病毒分离物进行分子生物学鉴定。结果从采集到的三带喙库蚊4400只和中华按蚊2200只中分离到9株阳性分离物,经分子生物学鉴定,7株分离物为流行性乙型脑炎(乙脑)病毒(JEV),均分离自三带喙库蚊;2株分离物为淡色库蚊浓核病毒(CppDNV),分离自三带喙库蚊和中华按蚊。结论首次在红河州弥勒县分离到乙脑病毒,从病原学证实弥勒县是乙脑流行区。流行株同源性和分子遗传进化分析证实为基因Ⅰ型乙脑病毒,为滇东南地区首次分离。Objective To investigate the distribution patterns of mosquito- borne viruses in Mile county, Honghe prefecture, Yunnan province, China, and to provide evidence for prevention and control of mosquito-borne diseases. Methods Mosquito samples were collected in Mile county during July 2009. Viruses were isolated from the samples through cell culture and then identified by molecular biology techniques. Results Nine virus strains were isolated from the mosquito samples including 4400 individuals of Culex tritaeniorhynchus and 2200 individuals of Anopheles sinensis. Seven strains, which were from Cx. tritaeniorhynchus, were identified as Japanese encephalitis virus （JEV）, and 2 strains, which were from Cx. tritaeniorhynchus and An. sinensis, were identified as Cx. pipiens pallens densovirus （CppDNV）. Conclusion JEV was first isolated from Mile county, Honghe prefecture, demonstrating that Mile county is an endemic area of JE from the etiological aspect. The prevalent JEV strain was confirmed to be genotype I, according to homology and phylogenetic analysis, and was first isolated in the southeast area in Yunnan province, China.

关 键 词：蚊虫 虫媒病毒 流行性乙型脑炎病毒 淡色库蚊浓核病毒 

分 类 号：R384.1[医药卫生—医学寄生虫学] R373.3[医药卫生—基础医学]