辽宁省汉滩型汉坦病毒基因特征及分布研究  

Genetic features and distribution of Hantaan virus in Liaoning province,China

作　　者：耿英芝[1] 田疆[1] 刘云[1] 王博[1] 孙英伟[1] 李鑫[1] 姚文清[1] 

机构地区：[1]辽宁省疾病预防控制中心感染与传染性疾病控制所,辽宁沈阳110005

出　　处：《中国媒介生物学及控制杂志》2012年第5期449-451,454,共4页Chinese Journal of Vector Biology and Control

基　　金：国家科技重大专项课题(2009ZX10004-209)~~

摘　　要：目的探讨辽宁省汉滩型汉坦病毒(HTNV)的基因特征及其分布情况。方法在辽宁省肾综合征出血热(HFRS)主要流行地区收集鼠肺和HFRS患者标本,采用间接免疫荧光法检测鼠肺中HV抗原,以RTPCR方法扩增标本中M和S基因片段的特异性核苷酸序列,将HTNV的阳性产物测序,并进行同源性比对和系统发生分析。结果辽宁省HFRS疫区HTNV主要为黑线姬鼠所携带;从鼠肺标本中扩增出M片段4份,患者标本中扩增出M片段5份,S片段1份;M片段的核苷酸同源性分析表明,辽宁株与Bao14、CJAp267等株同源性最高,为95%～97%,与HTNV原型株76-118同源性为87.4%～89.0%;系统发生分析显示辽宁株均分布于同一支内,与Bao14、CJAp267等株构成一个独立的支系,同属于H4亚型;TL2S基因片段与YaluRiver13核苷酸同源性最高(97.1%),与HTNV原型株76-118同源性为91.2%;推导的S片段氨基酸同源性,TL2与Bao14、CJAp93同源性较高,为93.0%～96.2%,与其他代表株同源性多在74.1%～81.6%;而且基于S片段的系统发生分析提示与M片段的分型结果基本一致,与Bao14、CJAp93等株位于同一分支内,为H4亚型。结论目前辽宁省流行的HTNV主要为H4基因亚型,基因亚型的分布相对比较单一。Objective To investigate the genetic features and distribution of Hantaan virus （HTNV） in Liaoning province, China. Methods Specimens from mouse lungs and patients with hemorrhagic fever with renal syndrome （HFRS） were collected in the high-epidemic areas of HFRS in Liaoning. The Hantavirus antigens in the mouse lung specimens were detected by indirect immunofluorescence assay. The specific nucleotide sequences of M and S segments in the specimens were amplified by reverse-transcription polymerase chain reaction （RT-PCR）. The positive PCR products of HTNV were subject to sequencing and then to homology analysis and phylogenetic analysis. Results The HTNV was carried mainly by Apodemus agrarius in the epidemic areas of HFRS in Liaoning. Four M segments were amplified from the mouse lung specimens, and five M segments and one S segment were amplified from the patient specimens. The homology analysis of the nucleotides of M segments showed that Liaoning strains shared the highest homology （95%-97%） with Baol4 and CJAp267, and had a homology of 87.4%-89.0% with HTNV prototype strain 76-118. The phylogenetic analysis of the M segments showed that the Liaoning strains, along with Baol4 and CJAp267, were in one branch of the phylogenetic tree, belonging to H4 subtype. The homology analysis of the nucleotides of S segment showed that TL2 demonstrated the highest nucleotide homology （97.1%） with YaluRiverl3, and had a homology of 91.2% with HTNV prototype strain 76-118. TL2 had homologies of 93.0%-96.2% with Baol4 and CJAp93 and 74.1%-81.6% with other representative strains, according to the amino acid homology deduction for S segment. The results of the phylogenetic analysis based on the S segment were approximately the same as those based on the M segments, suggesting that TL2, Baol4, and CJAp93 were in one branch of the phylogenetie tree. Conclusion H4 subtype HTNV is the most prevalent Hantavilus in Liaoning province, China, with few other genetic subtypes.

关 键 词：汉滩型病毒 基因亚型 系统发生分析 分布 

分 类 号：R373.3[医药卫生—病原生物学]