雷公藤甲素对人肝细胞L-02肝药酶活性的影响  

Effects of triptolide on hepatic drug metabolizing enzyme in human liver cell line L-02 cell

作　　者：姚金成[1] 刘颖[1] 胡领[2] 张陆勇[3] 饶健[1] 曾令贵[1] 江振洲[3] 何玲[3] 

机构地区：[1]湖南省药品审评认证与不良反应监测中心,湖南长沙410013 [2]中南大学湘雅二医院,湖南长沙410011 [3]中国药科大学国家新药筛选中心,江苏南京210009

出　　处：《中国医院药学杂志》2012年第20期1597-1600,共4页Chinese Journal of Hospital Pharmacy

基　　金：财政部行业科研专项(中药毒性的评价方法学研究和科学应用)资助课题(编号:200707008);湖南省科技厅科技计划项目(编号:2009FJ3027)

摘　　要：目的:研究雷公藤甲素对正常人肝细胞株L-02细胞的损伤作用及其对肝CYP3A及CYP2E1蛋白表达量的影响。方法:体外培养L-02细胞,MTT法检测雷公藤甲素对L-02细胞的损伤,试剂盒测定培养基上清液中AST、ALT及AKP活性,Western-blot测定细胞中CYP3A及CYP2E1蛋白的含量。结果:雷公藤甲素对L-02细胞有损伤作用,且随雷公藤甲素浓度的增加或孵育时间的延长,其损伤作用增强,同时,培养基中AST、ALT及AKP的活性增强;雷公藤甲素诱导细胞中CYP3A蛋白表达减弱,CYP2E1蛋白表达增强。结论:雷公藤甲素体外对人正常肝细胞L-02细胞有损伤作用,且能影响肝CYP450酶系的表达。OBJECTIVE To investigate the effects of triptolide on human liver cell L-02 in vitro and expression of CYP3A and CYP2E1. METHODS L-02 cells were cultured in vitro and the cytotoxic effects were detected by MTT. The activity of AST, ALT and AKP activities and expression of CYP3A and CYP2E1 were detected with commercial kits and Western-blot, respectively. RESULTS Triptolide had cytotoxic effects on human liver cell L-02 in ivro,markedly promoted accumulation of AST, ALT and AKP in culture medium. Triptolide significantly increased the concentration of MDA, the release of LDH and decreased the activity of SOD. CONCLUSION triptolide induced apoptosis of L-02 cells, associated with induction of ROS generation.

关 键 词：雷公藤甲素 人肝细胞株L-02 损伤 肝CYP450酶 

分 类 号：R285.5[医药卫生—中药学]