AGT、ACE基因多态性与成人过敏性紫癜和过敏性紫癜性肾炎的相关性  

Association of AGT and ACE gene polymorphism with genetic susceptibility to Henoch-Schonlein purpura and Henoch-Schonleinpurpura nephritis of adult

作　　者：安锦丹[1] 郭素芬[1] 赵洪涛[2] 金在顺[1] 颜彬[1] 曹永[1] 王洪伟[1] 

机构地区：[1]牡丹江医学院基础医学院病理教研室,157011 [2]牡丹江医学院基础医学院病理生理学教研室,157011

出　　处：《国际遗传学杂志》2012年第5期258-261,267,共5页International Journal of Genetics

基　　金：基金项目：牡丹江市科学技术计划项目（Z2011s003）;黑龙江省卫生厅科研项目（2011-322）

摘　　要：目的探讨肾素-血管紧张素系统中AGT基因M235T和ACE基因I／D多态性与过敏性紫癜和过敏性紫癜性肾炎易感性的关系。方法应用PCR和PCR—RFLP技术检测145例过敏性紫癜／过敏性紫癜性肾炎患者与172例正常对照组血管紧张素原基因第2外显子M235T多态性及血管紧张素转换酶基因第16内含子I／D多态性。结果①AGT基因型构成比在HSP组、HSPN组与正常对照组之间差异有统计学意义（P=0．008，P=0．002），但在HSP和HSPN之间差异无统计学意义（P=0．180）。AGT—TT基因型和丁等位基因携带者具有较高的患HSP、HSPN的风险。②ACE基因型构成比在HSPN组与正常对照组之间差异有统计学意义（P=0．003），但在HSP和正常对照组、HSP和HSPN之间差异无统计学意义（P=0．065，P=0．073）。ACE—DD基因型和D等位基因携带者具有较高的患HSPN的风险。结论携带AGT基因M235T多态性增加患HSP／HSPN的风险，携带ACE基因I／D多态性增加患HSPN的风险。Objective To explore M235 T polymorphism of angiotensinogen （AGT） gene and I/D polymorphism of angiotensin converting enzyme（ACE） gene in renin-angiotensin system, and their rela- tionship with Henoch-Sehonlein p＇urpura （ HSP ）/Henoch- Schonleinpurpura nephritis （ HSPN ）. Methods Insertion/deletion（I/D） polymorphism in intron 16 of ACE gene and number two exon M235T polymor- phisms of AGT gene were analyzed in 90 patients with HSP/HSPN and 98 healthy adult controls, by using PCR-RFLP and PCR. Results （1）The AGT genotype distribution was significantly different between HSP and normal control, and between HSPN and normal control （ P = 0. 008 , P = 0. 002 ） , but no difference was observed between HSP and HSPN （ P = 0. 180 ） . The AGT gene TT genotype and T allele has a high risk for HSP and HSPN. （2）The ACE genotype distribution was significantly different between HSPN and normal control （ P = 0. 003 ） , but no difference was observed between HSP and normal control, neither between HSP and HSPN（ P = 0. 065 ,P = 0. 073 ）. The ACE gene DD genotype and D allele has a high risk for HSPN. Conclusion AGT gene M235T polymorphism is associated with HSP/ HSPN. ACE gene I/D polymorphism is associated with HSPN.

关 键 词：过敏性紫癜 过敏性紫癜性肾炎 血管紧张素转换酶原 血管紧张素转换酶 多态性 

分 类 号：R554.6[医药卫生—血液循环系统疾病] R692.3[医药卫生—内科学]