城市地面交通现场暴露对雄性小鼠的生殖毒性  

Toxic Effect of Traffic Pollution on Reproductive System of Male Mice

作　　者：王征桦[1] 郑铃[1] 孙娜[1] 王章敬[1] Tong Lu 

机构地区：[1]福建医科大学公共卫生学院流行病学与统计学系,福建福州350004 [2]MayoC.linic, Department oflntemal Medicine,Rochester, Minnesota, USA 55905

出　　处：《环境与职业医学》2012年第10期632-634,637,共4页Journal of Environmental and Occupational Medicine

基　　金：福建省科技厅计划重点项目基金(编号:2007Y0017);福建医科大学教授学术发展基金(编号:JS10004)

摘　　要：[目的]探讨交通污染对雄性小鼠的生殖毒性作用。[方法]将30只雄性昆明系小鼠随机分为重度污染暴露组、轻度污染暴露组和清洁环境空白对照组,每组10只,进行现场暴露染毒,每日持续暴露10h。持续暴露35d后,观察交通污染对各组实验小鼠生殖器官发育、精子数量和质量、睾丸和附睾组织病理学变化以及睾丸细胞DNA损伤情况。[结果]与空白对照组小鼠相比,重度暴露组小鼠体重、睾丸和附睾重、睾丸脏器系数下降,睾丸细胞DNA损伤率增加;与空白对照组相比,轻度和重度暴露组小鼠精子数量下降,精子畸形率上升,差异均具有统计学意义(P<0.05)。组织病理学结果显示,轻度和重度暴露组小鼠睾丸和附睾组织有不同程度损伤。[结论]交通污染对雄性小鼠生殖系统会造成一定程度损伤。[ Objective ] To investigate the toxic effect of traiSc pollution on the reproductive system of male mice. [ Methods ] Thirty Kunming male mice were randomly divided into three groups （heavily polluted, lightly polluted, and blank control, 10 in each group）, and exposed to different traffic flows respectively for continuously 10h every day. After exposed for 35 d, development of genital organs, sperm density and abnormality, pathological changes of testis and epididymis, and DNA damage in testicular ceils were detected and analyzed. [ Results ] Compared with the blank control group, there were statistical differences in body weight, testis weight, epididymal weight, weight coemcient of testis, and DNA damage rate in testicular cells of the heavily polluted group （P 〈 0.05）; the sperm density was significantly higher and the abnormal sperm rate was significantly lower in both polluted groups （P 〈 0.05）. The histopathological results showed different levels of testicular damage in both the polluted groups. [ Conclusion ] Traffic pollution may damage male mice＇s reproductive system.

关 键 词：交通污染 睾丸 附睾 单细胞凝胶电泳 

分 类 号：R114[医药卫生—卫生毒理学] X51[医药卫生—公共卫生与预防医学]