维康醇对DNA拓扑异构酶活性的影响  

Effect of Alternol on DNA Topoisomerase Activity

作　　者：历文[1] 尹晓慧 段丽丽 陈杰鹏 周雪莹[1] 朱晓琳[1] 牛哲峰[1] 张美侠[1] 

机构地区：[1]中国医科大学药学院临床药理教研室,沈阳110001 [2]齐鲁制药有限公司研发部,济南250100 [3]广东汕头保税区双骏生物科技有限公司应用生物技术研究所,广东汕头515071

出　　处：《中国医科大学学报》2012年第10期889-891,共3页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(L2010565)

摘　　要：目的探讨维康醇对DNA拓扑异构酶活性的影响。方法以质粒pBR322超螺旋DNA为底物,采用凝胶电泳分别检测维康醇对拓扑异构醇Ⅰ(TopoⅠ)和拓扑异构醇Ⅱ(TopoⅡ)介导的pBR322 DNA解旋反应的影响。结果维康醇对TopoⅠ介导的pBR322解旋反应无明显的抑制作用,而对TopoⅡ的活性有明显的抑制作用。结论维康醇的直接作用靶点可能是DNATopoⅡ,该药可能成为新的TopoⅡ抑制剂。Objective To investigate the effect of Alternol on the activity of DNA topoisomerase.Methods The activity of TopoⅠ,Ⅱ was measured by TopoⅠ,TopoⅡmediated super coiled pBR322 relaxation.Results Alternol could significantly inhibit the activity of TopoⅡ,but had no effect on the activity of TopoⅠ.Conclusion Alternol can direct target DNA TopoⅡ in tumor,suggesting this drug may become a new TopoⅡ inhibitor.

关 键 词：维康醇 DNA拓扑异构酶 pBR322DNA 

分 类 号：R966[医药卫生—药理学]