天麻提取物N^6-羟苄腺嘌呤核苷对小鼠肌张力、运动协调能力及体温的影响  

Effect of N^6-(4-hydroxybenzyl) adenine riboside isolated from Gastrodia elata on muscle strength,motor coordination and body temperature in mice

作　　者：张莹[1] 石建功[1] 刘耕陶[1] 张建军[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所新药作用机制研究与药效评价北京市重点实验室,北京100050

出　　处：《国际药学研究杂志》2012年第5期420-424,共5页Journal of International Pharmaceutical Research

基　　金：国家重大新药创制科技重大专项(2012ZX09103-101-001);国家自然科学基金资助项目(30973512);国家杰出青年科学基金资助项目(30825044)

摘　　要：目的观察天麻提取物N6-羟苄腺嘌呤核苷(NHBA)对小鼠肌张力、运动协调能力及体温的影响。方法 ICR小鼠随机分为溶剂对照组、地西泮3 mg/kg组,以及NHBA 5和15 mg/kg组,分别经腹腔注射给药。应用转棒实验观察NHBA对小鼠运动协调能力的影响,应用悬挂实验观察NHBA对小鼠肌张力的影响,测定肛温观察NHBA对小鼠体温的影响,采用高效液相色谱-电化学检测法测定SD大鼠脑组织内单胺类神经递质含量。结果 NHBA 5和15 mg/kg对小鼠的肌张力无明显影响;在5 mg/kg剂量下,NHBA明显降低小鼠体温,给药100 min后体温恢复正常,对小鼠的运动协调能力无明显影响;在15 mg/kg剂量下,NHBA明显影响小鼠的运动协调能力,给药后140 min此作用消失。神经递质测定结果表明,NHBA 5 mg/kg可明显降低大鼠下丘脑、脑干单胺类神经递质去甲肾上腺素(NA)的含量。结论 NHBA具有降低小鼠体温、影响小鼠运动协调能力的作用,其对小鼠肌张力无明显影响。NHBA降低脑内促觉醒神经递质NA水平可能是其发挥中枢抑制作用的机制之一。Objective To investigate the effects ofN6（4hydroxybenzyl）adenine riboside （NHBA） on muscle strength, mo tor coordination and body temperature in mice. Methods ICR mice were randomly assigned into 4 groups ： vehicle control group, dia zepam 3 mg/kg group, NHBA 5 and 15 mg/kg groups. The effects of NHBA on muscle strength, motor coordination and body tempera ture in mice were observed in the traction test and rotarod test. The contents of monoamine neurotransmitters in rat brain were deter mined by high performance liquid chromatographyelectrochemical detection. Results The results showed that intraperitoneal adminis tration of NHBA （5 and 15 mg/kg） had no effect on muscle strength, but NHBA reduced body temperature at dose of 5 mg/kg, and body temperature returned to normal 100 rain after administration. NHBA had no effect on motor coordination at dose of 5 mg/kg, but at dose of 15 mg/kg, it significantly interfered with motor coordination in mice, and its action disappeared 140 min after administra tion. The contents of norepinephrine in hypothalamus and brain stem of rats were decreased significantly. Conclusion NHBA can im pair motor coordination and reduce body temperature in mice. Meanwhile it can decrease contents of norepinephrine in hypothalamus and brain stem, which may be involved in the central inhibitory activity of NHBA.

关 键 词：天麻 N6-羟苄腺嘌呤核苷 运动协调能力 肌张力 体温 单胺类神经递质 

分 类 号：R285[医药卫生—中药学]