重楼皂苷I对高转移人卵巢癌细胞体外生长抑制功能的研究  

Research on Proliferation Inhibitory Effect of Paris Saponin I on High Metastatic Human Ovarian Cancer Cell Line HO-8910PM in vitro

作　　者：顾琳慧[1] 冯建国[1] 钱丽娟[1] 马胜林[2] 

机构地区：[1]浙江省肿瘤研究所,浙江杭州310009 [2]杭州市第一人民医院,浙江杭州310022

出　　处：《中华中医药学刊》2012年第10期2212-2215,I0003,I0004,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药重点资助项目(2011ZZ011);浙江省自然科学青年基金资助项目(LQ12H16015)

摘　　要：目的:研究重楼提取的活性成分重楼皂苷I对高转移人卵巢癌细胞的体外生长抑制及促凋亡功能,并初步探讨其可能的作用机制。方法:用CCK8法检测不同浓度重楼皂苷I在不同时间点对高转移人卵巢癌细胞(HO-8910PM)体外增殖的影响;光镜下观察细胞形态的变化;用流式细胞术(FCM)检测经不同浓度重楼皂苷I处理后高转移人卵巢癌细胞的细胞周期变化;用AnnexinV/PI双染法、Hoechst33258检测不同浓度重楼皂苷I诱导细胞凋亡的发生。结果:重楼皂苷I在0.5～5μg/mL浓度范围对HO-8910PM细胞的增殖均有明显抑制作用,并呈现明显的剂量-时间依赖性。其半数抑制浓度在24h到120h范围内基本集中在1～3.5μg/mL之间;重楼皂苷I主要影响HO-8910PM细胞的DNA合成前期(G0/G1);重楼皂苷I浓度控制在2μg/mL以内,早期即3h开始就能诱导HO-8910PM细胞的凋亡,作用时间越长,凋亡发生率越高。结论:重楼皂苷I能抑制卵巢癌细胞的增殖并诱导其凋亡,从而发挥抗肿瘤作用。Abstract：Objective：To investigate the proliferation inhibitory and apoptosis effect of Paris Saponin I（PSI）, an active component isolated from Rhizoma Paridis, on high metastatic ovarian cancer cell line HO -8910PM in vitro, and explore the possible mechanisms. Methods：We dealed with the cell line HO -8910PM at different times with different concentra, tions of PSI. CCK8 assay was used to examine the status of cell growth. Changes in cell morphology were observed underoptical microscope. Flow cytometry, AnnexinV/PI double -staining and Hoechst33258 methods were adopted to deter- mine cell cycle and apoptosis changes. Results ：Different concentrations of PSI （0.5 ～5 μg/mL） all showed markedly inhib- itory effect in a dose -dependent manner on HO -8910PM ,especially in GO/G1 phase. The concentrations to inhibit cell growth by 50% （IC50） were 1 ～3.5 μg/mL from 24 to 120h. Early use of PSI （ at the beginning of 3 h, 〈 2 μg/mL） could induce apoptosis. Conclusion：PSI can inhibit the metastatic ovarian cancer cell line growth,induce apoptosis, and thereby resist carcinoma.

关 键 词：重楼皂苷I 卵巢肿瘤 细胞系 凋亡 

分 类 号：R285.5[医药卫生—中药学]