健肝颗粒对大鼠肝纤维化脂质过氧化物生成的影响  

Effects of Jiangan Granules on Lipid Peroxidation in Rats with Liver Fibrosis

作　　者：黄古叶[1] 龙富立[1] 毛德文[1] 邱华[1] 石清兰[1] 刘茵[1] 

机构地区：[1]广西中医学院第一附属医院肝病科广西中医肝病治疗中心,广西南宁530023

出　　处：《中华中医药学刊》2012年第10期2232-2234,I0005,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：广西教育厅科研资助项目(桂教计字201012MS149)

摘　　要：目的:采用经典的CCL4复合因素建立肝纤维化大鼠模型,探究健肝颗粒抗肝纤维化的作用机制。方法:100只雄性SD大鼠随机分组后,以CCL4腹腔内注射的方法建立大鼠肝纤维化模型,造模后施以由低、中、高剂量健肝颗粒,以秋水仙碱为阳性对照。比色法检测血清活性氧(ROS)水平;硫代巴比妥酸(TBA)法检测肝组织中匀浆中丙二醛(MDA)含量。结果:模型组大鼠血清ROS水平明显升高,健肝颗粒可显著降低ROS水平,尤其以低剂量组效果明显(P<0.05);健肝颗粒组大鼠肝组织中MDA含量明显低于模型组(P<0.01)。结论:健肝颗粒可抑制肝脏脂质过氧化物的形成,可能是其抗肝纤维化的作用机制之一。Objective ： To investigate the mechanism underlying the possible anti - fibrotic effects of Jiangan granules on rats with carbon tetrachloride（CC14） induced liver fibrosis. Method~：One hundred male SD rats with liver fibrosis induced by intraperitoneal injection of CC14 were randomized to receive a low, intermediate, or high dose of Jiangan granules or the positive control colchicine. Serum reactive oxygen species（ROS） were measured colorimetrically. The levels of malondial- dehyde（MI）A） in liver tissue homogenates were assessed by the thiobarbituric acid （TBA）assay. Results：Rats receiving colchicine had significantly increased levels of ROS. In contrast, Jiangan granules was associated with a significant de- crease in ROS, especially the low dose （P 〈 0.05 ）. Compared with the controls, rats given Jiangan granules had signifi- cantly lower levels of MDA（ P 〈 0.01 ）. Conclusions ： Jiangan granules appear to gain an anti - fibrotic effect by inhibiting the production of lipid peroxides in the liver.

关 键 词：肝纤维化 健肝颗粒 脂质过氧化作用 机制研究 

分 类 号：R285.5[医药卫生—中药学]