清开通灌肠液对内毒素性发热兔的退热效应及对血管活性肠肽、白介素-2的影响  

Study on the Antipyretic Effection of Qingkaitong Enema and Its Influence on the Changes of Vasoactive Intestinal Peptide(VIP)and Interleukin-2(IL-2)in Endotoxin-induced Febrile Rabbits

作　　者：刘亚娟[1] 张思超[1] 王晓君[1] 孙敬昌[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《中华中医药学刊》2012年第10期2234-2236,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：山东省中医药科技发展计划资助项目(2005-124)

摘　　要：目的:观察基于"脑-肠轴"学说组方的清开通灌肠液,对内毒素性发热兔的退热效应及VIP、IL-2在发热中的变化。方法:日本大耳白家兔60只,随机分成4组:正常组、模型组、清开灵组和清开通组。模型组、清开灵组及清开通组,各组经耳缘静脉注射无热源生理盐水稀释的内毒素,建立兔内毒素性发热模型。同时清开灵组、清开通组分别予以清开灵、清开通灌肠液灌肠。注射内毒素2h后,清开灵组、清开通组再予灌肠1次。4h后,各组动物随机抽取10只,采集脑、肠及外周血标本,用放射免疫法检测脑、肠及血浆中VIP、IL-2含量。持续观察每组另外5只动物体温变化情况,每间隔10min记录1次体温,连续测温12h。结果:清开灵组和清开通组动物的体温与模型组相比都有明显降低(P<0.01),但清开通组降低比清开灵组更为显著(P<0.05)。脑肠及血浆中VIP和IL-2的含量都显著低于模型组(P<0.01),尤其清开通组较清开灵组降低幅度更大,二者有显著差异(P<0.05)。结论:"清开"与"通腑"合治,较单一清开法在退热及提高机体免疫方面效果更著,VIP和IL-2在发热中起正调节作用。Abstract：Objective：To investigate the antipyretic effect of Qingkaitong enema according to the theory of brain gut axis in endotoxin induced febrile rabbits, and observe changes of vasoactive intestinal peptide （VIP） and interleukin 2 （ IL 2） in different tissues. Methods ： Sixty Japanese White Rabbits were randomly divided into following four groups ： nor mal control group, model group, Qingkailing enema group and Qingkaitong enema group. The Eseherichia coli endotoxinswere injected into marginal ear veins of the rabbits of model group, Qingkailing enema group and Qingkaitong enema group to induce the rabbit febrile model. Qingkailing enema group and Qingkaitong enema group were immediately treated with Qingkailing enema and Qingkaitong enema respectively after the Escherichia coli endotoxins were injected. Qingkailing enema group and Qingkaitong enema group were once again treated with Qingkailing enema and Qingkaitong enema re spectively after two hours. The brain, intestine and peripheral blood samples were obtained from ten rabbits which were selected randomly in every group. The levels of vasoactive intestinal peptide （VIP）and interleukin 2 （IL 2 ）were detec ted by radioimmunoassay. The body temperatures of the remaining five rabbits in every group were taken every ten mi nutes for 12 hours. Results：The rabbits temperatures of Qingkailing enema group and Qingkaitong enema group were obvi ously lower than that of the model group（ P 〈0.01 ）. Qingkaitong enema group had lower temperature significantly（ P 〈 0.05） ,compared with Qingkailing group. The levels of vasoactive intestinal peptide（VIP） and interleukin 2（IL2）in different tissues of Qingkailing enema group and Qingkaitong enema group were decreased evidently, compared with the model group（ P 〈 0. O1 ）. Qingkaitong group had lower levels of vasoactive intestinal peptide（VIP） and interleukin 2（ IL 2） than Qingkailing group did （ P 〈 0.05 ）. Conclusion ： The effects of Qingkai and Ton

关 键 词：清开通灌肠液 发热 VIP IL-2 兔 

分 类 号：R285.5[医药卫生—中药学]