阿糖尿苷探针同步荧光法快速测定生物样品中的蛋白含量  

Study of synchronous fluorescence technique with spongouridine as probe for rapid detection of proteins in biological samples

作　　者：余望舒[1] 张运成[2] 崔凤灵[1] 惠广全[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007 [2]安阳职业技术学院化工系,安阳455008

出　　处：《分析试验室》2012年第11期18-20,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金资助项目(30970696)资助

摘　　要：通过研究阿糖尿苷与人血清白蛋白(HSA)体系的三维荧光光谱,证明两者可以发生相互作用,并导致HSA的微环境和构象发生变化。考查了Δλ、加入顺序、离子强度等因素对该体系同步荧光强度的影响,选取阿糖尿苷测定生物样品中蛋白含量的最佳实验条件。体系的同步荧光强度与HSA的浓度在0.04～4.0×10-6mol/L范围内呈良好的线性关系,此法的检测限可达0.75 mg/L,线性相关系数为0.9990。在此基础上,以阿糖尿苷为分子探针,测定了人血清、唾液和尿样中的蛋白质含量,回收率在97.3%～104.5%之间。The experiment is investigated for three-dimensional fluorescence spectrum. The results showed that spongouridine can change the micro-environment and conformation of human serum albumin （HSA）. With the consideration of the effect factors for spectral characterization and intensity of synchronous fluorescence, such as the value of △λ, addition sequence, ionic strength and so on, the optimum conditions of this experiment are selected. HSA has a good linear relationship in the range of 0.04 -4. 0 × 10-6 tool/L, with the detection limit of 0. 75 mg/L. The linear correlation coefficient is 0. 9990. This method has been; applied to the determination of the proteins in human serum, saliva and urine samples, and the recoveries are 97.3% × 104.5%.

关 键 词：阿糖尿苷 人血清白蛋白 三维荧光光谱 同步荧光光谱 分子探针 

分 类 号：O657.3[理学—分析化学]