结合近红外光谱法的肝血流参数测量  

Measurement of liver blood flow parameters combined with near-infrared spectroscopy

作　　者：张晓枫[1,2] 刘光达[1] 辛桂杰[3] 焦阳[1] 徐国忠[2] 

机构地区：[1]吉林大学仪器科学与电气工程学院,吉林长春130061 [2]长春理工大学生命科学技术学院,吉林长春130022 [3]吉林大学第一医院,吉林长春130061

出　　处：《光学精密工程》2012年第10期2140-2146,共7页Optics and Precision Engineering

基　　金：吉林省科技厅资助项目(No.20120328)

摘　　要：针对常规的色素浓度测量方法存在操作复杂、有创和非连续等问题,研究了基于色素稀释和排泄试验的脉搏光谱分析方法。通过静脉注射吲哚青绿指示剂,选定两特定波长的近红外光作为吲哚青绿色素吸光度测量点,并采用指夹式传感器提取人体指尖末梢血的光谱信号,通过连续记录色素浓度稀释曲线,实现了分离吲哚青绿浓度谱和脉搏血氧吸收光谱的算法。最后分析呈指数衰减规律变化的半对数坐标色素浓度图,完成了色素血浆消失率K和15min滞留率R15等肝血流参数的无创测量。该方法基于Lab-VIEW平台,结合自适应差分阈值法,有效剔除了血红蛋白吸收光谱和基线漂移的干扰,各项参数检测准确率高,K值平均测量误差小于0.008。该检测理论和方法既可用于临床肝切除手术的术前、术中和术后的肝储备功能检查,也可为医学研究提供相应的中间测量过程及中间观察变量。Abstract： A pulse spectroscopic scheme based on dye dilution and an excretion test was developed to overcome the shortcomings of conventional blood dye concentration measuring method in complex operation, invasive treatment and non-continuance. Firstly, Indocyanine Green（ICG） was intravenously injected and a finger clip sensor was used to extract spectral signals of fingertips for a sufferer. Then, by recording the dye dilution curve simultaneously , the algorithm to isolate ICG absorption spectrum from the disturbance of peripheral arterial blood was implemented. Finally, the ICG plasma disappearance rate K, 15 min retention rate R15 and other hepatic hemodynamic parameters were deduced by analyzing the semi-logarithmic dye concentration graph. Combining a lab-VIEW software platform with an adaptive differential threshold method, proposed scheme eliminates the interference come from the hemoglobin absorption spectrum and baseline drift, improves the measuring accuracy greatly, and the average measurement error of K is less than 0. 008. This method is great value to not only the assessing hepatic functional reserve at pre-operation, intra-operation, post-operation, but also providing a way to monitor some middle variables for clinical research purposes.

关 键 词：吲哚青绿 色素浓度谱 血红蛋白 红外吸收光谱 脉搏波 肝血流参数 

分 类 号：O657.33[理学—分析化学] R318[理学—化学]