电击损伤后大鼠CK-MB及HSP 60的水平变化  

Changes of CK-MB and HSP 60 in Electrical-Injuried Rats

作　　者：刘慧通[1] 付高文 赵泽[1] 丁素真[1] 王乔峰[1] 陈蕾[1] 谢亚男[1] 王振原[1] 

机构地区：[1]西安交通大学医学院,陕西西安710061 [2]白水县公安局,陕西白水715600

出　　处：《法医学杂志》2012年第5期333-336,共4页Journal of Forensic Medicine

基　　金：国家大学生创新性实验计划资助项目(091069868)

摘　　要：目的观察无电流斑电击伤后大鼠血清肌酸激酶同工酶（creatine kinase—MB．CK—MB）及热休克蛋白60（heat shock protein60，HSP60）的变化水平，探讨其变化与电流损伤时间的相关性，评估电击后相应组织细胞损伤情况。方法利用110V交流电电击大鼠，建立大鼠无电流斑电击损伤模型。取大鼠的血液进行血清CK—MB全自动生化检测，心肌和肝左叶组织进行HSP60免疫组织化学染色。结果电击组大鼠血清CK—MB在电击损伤早期即升高，在30min达到峰值，此后递减，3～5h下降趋势减缓，于电击后6h趋于平稳。免疫组织化学染色显示，电击后大鼠心肌和肝细胞中HSP60表达水平产生一定的规律性改变。结论电流损伤后大鼠血清CK—MB和组织HSP60水平发生一定的规律性改变．对于电击损伤的诊断和电击后内部器官损伤的评估具有辅助意义。Objective To investigate the changes of creatine kinase-MB （CK-MB） and heat shock protein 60 （HSP 60） in rats without electric marks after electric injury, to identify the relationship of the CKMB, HSP 60 and the time of electric injuries, and to evaluate the damage to cells after electric injury. Methods The animal model of electric injury without electric marks was established by alternating current （voltage 110V）. Automatic biochemistry analyzer was used to detect the serum CK-MB and immunohisto-chemical staining technology was used to analyze the tissues of myocardium and left lobe of liver. Results The amount of serum CK-MB was increased when the rats were injuried, and reached the peak at 30min. Then the amount of CK-MB began to decrease and showed a slight downward trend in 3-5 h after electric injury, and leveled off at 6 h. Immunohistochemistry staining also showed the changes of HSP 60 of rats＇ myocardial cells and hepatic cells regularly after electric injury. Conclusion The regular changes of serum CK-MB and tissular HSP 60 in rats can be used to diagnosis electric injury and assess the injury of internal organs after the electric injury without electric marks.

关 键 词：法医病理学 电击伤 肌酸激酶 MB型 热休克蛋白质类 大鼠 

分 类 号：DF795.1[医药卫生—法医学]