外周血和月经血鉴别的新方法  

A New Method of Identifying the Peripheral Blood and the Menstrual Blood

作　　者：王颖希[1] 朱晓君[2] 焦章平[2] 路志勇[2] 王伟妮[1] 刘雅诚[2] 唐晖[2] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]北京市公安局刑事侦查总队,北京100192

出　　处：《法医学杂志》2012年第5期359-361,共3页Journal of Forensic Medicine

基　　金：北京市科技计划课题(Z111100056811024)

摘　　要：目的从基因表达的组织特异性入手,寻找外周血和月经血的特异性指标,并建立对月经血和外周血进行鉴别的新方法。方法从检材中提取RNA后进行RT-PCR,通过电泳分离检测特异性的产物。结果外周血特异性的标志物膜收缩蛋白β(beta-spectrin,SPTB)和管家基因18S核糖体RNA(18S rRNA)在外周血及月经血中均表达;月经血特异性的标志物基质金属蛋白酶7(matrix metalloproteinase 7,MMP7)和阴道分泌物特异性的标志物人β防御素1(human beta defensin 1,HBD1)只在月经血中表达。结论外周血和月经血的基因表达存在差异,利用荧光标记结合RT-PCR检测特异性mRNA标志物的方法可以区分两者。Objective To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the pe- ripheral blood and the menstrual blood. Methods The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by eleetrophoretic technology. Results Betaspectrin （SPTB） as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 （MMPT） as one specific marker of menstrual blood and human beta defensin 1 （HBD1） as one specific marker of vaginal discharge were only found in the menstrual blood. Conclusion There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA.

关 键 词：法医遗传学 月经血 外周血 鉴别 

分 类 号：DF795.2[医药卫生—法医学]