MEBT/MEBO对皮肤创面愈合过程中VEGF、bFGF、EGF mRNA表达影响的研究  

The effect of MEBT/MEBO on the expression of VEGF,bFGF and EGF mRNAs during skin wound healing

作　　者：唐乾利[1] 韩珊珊[2] 付军[3] 代波[4] 冯婧[4] 黄欣[4] 李杰辉[3] 狄钾骐[3] 

机构地区：[1]右江民族医学院,广西百色533000 [2]广西中医药大学附属瑞康医院整形美容中心,广西南宁530011 [3]广西中医药大学第一附属医院,广西南宁530023 [4]广西中医药大学,广西南宁530001

出　　处：《右江民族医学院学报》2012年第5期597-601,共5页Journal of Youjiang Medical University for Nationalities

基　　金：2008年国家自然科学基金项目(30860356)

摘　　要：目的探讨MEBT/MEBO促进创面愈合的作用机制。方法建立大鼠急性损伤创面模型,观察MEBT/MEBO对模型创面组织VEGF、bFGF、EGF mRNA表达的影响。结果①创面肉芽组织生长大体情况:造模后第8天,MEBT/MEBO组、贝复济组大鼠创面肉芽组织生长情况明显好于模型组,MEBT/MEBO组和贝复济组大鼠创面较红润,可见散在的肉芽颗粒生长,模型组大鼠创面较苍白,生长缓慢。②愈合时间:MEBT/MEBO组大鼠创面平均愈合时间为(12.50±0.83)天,短于贝复济组(13.15±0.88)天,明显短于模型组(14.20±1.28)天,MEBT/MEBO组与贝复济组、模型组在愈合时间方面相比差异均有显著性(P<0.05,P<0.01)。③VEGF、bFGF、EGF mRNA表达:模型组大鼠的表达量在造模后8天时相点显著低于MEBT/MEBO组、贝复济组(P<0.01),MEBT/MEBO组在造模后8天时相点比贝复济组的表达量稍高,差异有显著性(P<0.05)。结论①MEBT/MEBO可以提高大鼠肉芽组织中VEGF、bFGF、EGFmRNA的表达水平,推测MEBO可能通过对VEGF、bFGF、EGF的调控,促进创面成纤维细胞的分裂增殖及新生毛细血管的增殖,从而促进肉芽组织形成,加速创面愈合。②MEBT/MEBO能明显缩短实验性SD雄性大鼠体表创伤创面修复愈合时间,具有促进大鼠体表创伤创面愈合的作用。To explore the mechanism of MEBT/MEBO in promoting wound healing.Rat models of acute lesion were established, and the effect of MEBT/MEBO on the expression of VEGF, bFGF and EGF mRNAs in the wound models was studied. Results （I） Growth of granulation tissue in the wounds： 8 daysafter the modelling, granulation tissues in MEBT/MEBO and Beifuji Groups grew much better than that in Model Group, wound surfaces of MEBT/MEBO and Beifuji Groups were red and showed scattered granulation whereas that of Model Group was relative pale and had little granulation. ~） Wound duration （the duration between getting wound and healing）： The average wound duration of MEBT/MEBO Group （12.50 ＋ 0.83） days was shorter than that of Beifuji Group （13.15 ＋ 0.88） days and Model Group （14.20 ＋ 1.28） days, the differences among MEBT/MEBO and Beifuji and Model Groups being statistically significant （ P 〈 0.05, P 〈 0.01 ）. Expressions of VEGF, bFGF and EGF mRNAs： 8 days after the modelling, the expression levels of VEGF, bFGF and EGF mRNAs in rats of Model Group was notably lower than that in rats of MEBT/MEBO and Beifuji Groups （ P 〈 0.01）, and that in rats of MEBT/MEBO Group was slightly higher than that in rats of Beifuji Group （ P 〈 0.05）. Conelusion （~） MEBT/MEBO can enhance the expression level of VEGF, bFGF and EGF mRNAs in granulation tissue of rats; it may promote the formation of granulation tissue and speed up wound healing by regulating VEGF, bFGF and EGF and increasing proliferation fibroblasts and new blood capillaries in the wound. MEBT/MEBO can markedly short- en the wound duration of experimental male SD rats, suggesting that it has the effect of promoting wound healing in rats.

关 键 词：创伤与损伤 投药 皮肤 MEBT/MEBO 创面愈合 VEGF MRNA BFGFMRNA EGF MRNA 

分 类 号：R641[医药卫生—外科学]