鸿雁源鸭甲肝炎病毒Ⅰ型的分离与鉴定  

ISOLATION AND INDENTIFICATION OF DUCK HEPATITIS A VIRUS TYPE Ⅰ FROM A SWAN GOOSE(ANSER CYGNOIDES)

作　　者：施少华[1,2] 陈红梅[1,2] 陈珍[1] 傅光华[1,2] 万春和[1,2] 程龙飞[1,2] 黄瑜[1,2] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013 [2]福建省畜禽疫病防治工程技术研究中心,福州350013

出　　处：《中国动物传染病学报》2012年第5期55-59,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家公益性行业科研专项(201003012);福建省自然科学基金(2011J01113);现代农业产业技术体系建设专项资金(CARS-43);福建省"种业创新与产业化工程建设项目"(2011FJZY-9)

摘　　要：为了对一起死亡率高达91．3％、急性死亡的鸿雁病例进行病原学分析，通过细菌分离排除法和病毒分离方法获得致鸭胚死亡病毒（暂命名为FJ-017株）。该病毒无血凝活性，用鹅细小病毒、番鸭细小病毒、鸭呼肠孤病毒、鸭甲肝炎病毒1型、鸭瘟病毒、鸭坦布苏病毒特异引物分别进行扩增，电泳结果显示，仅鸭甲肝炎病毒1型引物可扩增出条带。将扩增产物回收后克隆，序列分析表明FJ-017株与22株DHAV-1参考株的同源率为93．5％-99％，而与DHAV-2和DHAV-3参考株的同源性均为79．9％。遗传进化分析表明FJ-017株与DHAV-1关系密切，在进化树中共处一分支，表明FJ-017株为鸭甲肝炎病毒1型，此为国内外首次报道。The outbreak of an acute disease caused 91.3% fatality in a swan goose flock in Fujian Province. A Duck hepatitis A virus （DHAV） was isolated from a dead swan goose and designated as FJ-017 strain. The isolated FJ-017 strain had no hemagglutination activity. The genome of FJ-017 strain was amplified in PCR using specific primers targeted for DHAV-1 but not for Goose parvovirus, Duck parvovirus, Duck reovirus, Duck enteritis virus and Duck tembusu virus. Sequence analysis revealed high homology （93.5%-99%） between F J-017 strain and 22 DHAV-I strains in GenBank. The relatively lower homology （79.9%）, however, existed between F J-017 strain and two reference strains of DHAV-2 and DHAV-3. Phylogenetic analysis showed that F J-017 strain and DHAV-1 reference strains belonged to a distinct cluster. This was the first isolation of DHAV-1 virus from a swan goose.

关 键 词：鸿雁 鸭甲肝炎病毒 分离鉴定 

分 类 号：S858.32[农业科学—临床兽医学]