巴西橡胶树HbNAC1基因启动子的克隆及序列分析  

Cloning and Sequence Analysis of HbNAC1 Promoter from Hevea brasiliensis

作　　者：康桂娟[1] 黎瑜[1] 夏可灿[1,2] 聂智毅[1] 曾日中[1,2] 

机构地区：[1]中国热带农业科学院橡胶研究所,农业部橡胶树生物学重点开放实验室,省部共建国家重点实验室培育基地,儋州571737 [2]海南大学农学院,海口570228

出　　处：《基因组学与应用生物学》2012年第5期447-454,共8页Genomics and Applied Biology

基　　金：中国热带农业科学院橡胶研究所基本科研业务费专项资金(1630022011024);国家自然科学基金项目(31201143)共同资助

摘　　要：NAC转录因子是植物特有的一类转录调控因子,在植物的生长发育、激素调节和境胁迫应答中具有重要的功能。为研究NAC转录因子在巴西橡胶树(Hevea brasiliensis)抗逆胁迫中的功能,本项目组根据巴西橡胶树NAC转录因子-HbNAC1基因序列,通过Genome Walking方法从巴西橡胶基因组DNA中获得了长度为1861bp的HbNAC1基因的5'调控区片段,序列分析表明该段序列含有一个典型的真核生物核心启动子区域,转录起始位点T位于起始密码子上游52bp处。该启动子序列除了含有TATA-box、CAAT-box等基本顺式作用元件外,还具有茉莉酸响应元件以及大量光顺式作用元件和逆境胁迫诱导相关的顺式调控元件,这表明HbNAC1基因在橡胶树逆境胁迫应答过程具有重要功能,其启动子可能是一个光诱导型和组织特异性启动子。Plant-specific NAC transcription factors play important roles in plant growth and development, hormone regulations and stress responses. In order to study the roles of NAC transcription factor playing on rubber tree resistance to various stresses, a 1 681 bp 5＇ regulatory sequence of HbNAC1 was cloned from leaf genomic DNA of Hevea brasiliensis based on the the cDNA sequences using Genome Walking method. Bioinformation analysis indicated that there was a typical core promoter region of eukaryotic, and the transcription start site （TSS, T） located 52 bp upstream of the initiation codon ATG. The promoter contains the basic elements： TATA-box, CAAT-box, and a MeJA-responsive elements as well as multiple light-responsive elements and stress-induced elements. It is suggested that HbNAC1 gene may play important roles in response to various stresses in rubber tree, and the promoter might be light-inducible and tissue-specific promoter.

关 键 词：巴西橡胶树 HbNAC1启动子 顺式调控元件 逆境胁迫 

分 类 号：S794.1[农业科学—林木遗传育种]